Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-6284
Authors: Wölfinger, Pascal
Epp, Katharina
Schaefer, Lukas
Kriege, Diana
Theobald, Matthias
Bopp, Tobias
Wagner-Drouet, Eva-Maria
Title: CD52-negative T cells predict acute graft-versus-host disease after an alemtuzumab-based conditioning regimen
Online publication date: 16-Aug-2021
Language: english
Abstract: Allogeneic haematopoietic stem cell transplantation (HSCT) after a reduced-intensity conditioning (RIC) regimen with fludarabine, melphalan and alemtuzmab is an effective therapy for haematological malignancies. Alemtuzumab, a monoclonal antibody against CD52, a glycosylphosphatidylinositol-anchor-bound surface protein on lymphocytes, depletes T cells to prevent graft-versus-host disease (GVHD). Despite this, acute and chronic GVHD (a/cGVHD) remain life-threatening complications after HSCT. The aim of the present study was to identify parameters to predict GVHD. In 69 patients after HSCT, T-cell subsets were functionally analysed. Reconstitution of CD52neg T cells and CD52neg regulatory T cells (Tregs) correlated with onset, severity and clinical course of aGVHD. Patients with aGVHD showed significantly lower levels of CD52pos T cells compared to patients with cGVHD or without GVHD (P < 0·001). Analysis of T-cell reconstitution revealed a percentage of <40% of CD52posCD4pos T cells or CD52pos Tregs at day +50 as a risk factor for the development of aGVHD. In contrast, CD52neg Tregs showed significant decreased levels of glycoprotein A repetitions predominant (GARP; P < 0·001), glucocorticoid-induced TNFR-related protein (GITR; P < 0·001), chemokine receptor (CXCR3; P = 0·023), C-C chemokine receptor type 5 (CCR5; P = 0·004), but increased levels of immunoglobulin-like transcript 3 (ILT3; P = 0·001), as well as a reduced suppressive capacity. We conclude that reconstitution of CD52neg T cells and CD52neg Tregs is a risk factor for development of aGVHD.
DDC: 610 Medizin
610 Medical sciences
Institution: Johannes Gutenberg-Universität Mainz
Department: FB 04 Medizin
Place: Mainz
DOI: http://doi.org/10.25358/openscience-6284
Version: Published version
Publication type: Zeitschriftenaufsatz
License: CC-BY-NC
Information on rights of use: https://creativecommons.org/licenses/by-nc/4.0/
Journal: British journal of haematology
191
2
Pages or article number: 253
262
Publisher: Wiley-Blackwell
Publisher place: Oxford u.a.
Issue date: 2020
ISSN: 1365-2141
Publisher's URL: https://doi.org/10.1111/bjh.16706
Appears in collections:JGU-Publikationen

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