Authors:	Wagener, Karolin Bros, Matthias Krumb, Matthias Langhanki, Jens Pektor, Stefanie Worm, Matthias Schinnerer, Meike Montermann, Evelyn Miederer, Matthias Frey, Holger Opatz, Till Rösch, Frank
Title:	Targeting of immune cells with trimannosylated liposomes
Online publication date:	13-Aug-2021
Year of first publication:	2020
Language:	english
Abstract:	Dendritic cells (DCs) are a compelling target in cancer immunotherapy as they initialize strong antigen-specific immune responses. Drug delivery systems (DDSs) such as liposomes provide the opportunity to deliver antigens and immunostimulatory molecules to DCs, which in turn initiate an antigen-specific immune response. To address predominantly DCs, DDSs need to be equipped with targeting moieties. This study evaluates liposomes, bearing the oligosaccharide trimannose on their surface, for their ability to address DCs in vitro and in vivo. Trimannose as a saccharidic structure is known to be recognized by receptors on the surface of DCs. To obtain trimannosylated liposomes, azide-bearing trimannose is coupled to alkyne-functionalized hyperbranched polyglycerol (hbPG) with a bis(hexadecyl)glycerol (BisHD) anchor in a Cu(I)-catalyzed alkyne-azide cycloaddition (CuAAC). To enable tracking of the liposomes in vivo, the trimannosylated BisHD-hbPG lipids are radiolabeled with 18F in a CuAAC. Subsequently, liposomes are produced via the thin-film hydration method followed by extrusion. The behavior of the trimannosylated liposomes is evaluated in in vitro cell binding assays and in vivo µPET and ex vivo biodistribution studies in healthy C57BL/6 mice and the results are compared to similar liposomes not bearing trimannose on their surface.
DDC:	540 Chemie 540 Chemistry and allied sciences 570 Biowissenschaften 570 Life sciences
Institution:	Johannes Gutenberg-Universität Mainz
Department:	FB 09 Chemie, Pharmazie u. Geowissensch.
Place:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-6272
Version:	Published version
Publication type:	Zeitschriftenaufsatz
License:	CC BY-NC-ND
Information on rights of use:	https://creativecommons.org/licenses/by-nc-nd/4.0/
Journal:	Advanced therapeutics 3 6
Pages or article number:	1900185
Publisher:	Wiley-VCH
Publisher place:	Weinheim
Issue date:	2020
ISSN:	2366-3987
Publisher URL:	https://doi.org/10.1002/adtp.201900185
Publisher DOI:	10.1002/adtp.201900185
Appears in collections:	JGU-Publikationen