Autoren:	Stengelin, Elena Kuzmina, Alena Beltramo, Guillermo L. Koziol, Martha F. Besch, Laura Schröder, Romina Unger, Ronald E. Tremel, Wolfgang Seiffert, Sebastian
Titel:	Bone scaffolds based on degradable vaterite/PEG-composite microgels
Online-Publikationsdatum:	9-Aug-2021
Erscheinungsdatum:	2020
Sprache des Dokuments:	Englisch
Zusammenfassung/Abstract:	Vaterite, a metastable modification of calcium carbonate, embedded in a flexible microgel packaging with adjustable mechanical properties, functionality, and biocompatibility, provides a powerful scaffolding for bone tissue regeneration, as it is easily convertible to bone-like hydroxyapatite (HA). In this study, the synthesis and physical analysis of a packaging material to encapsulate vaterite particles and osteoblast cells into monodisperse, sub-millimeter-sized microgels, is described whereby a systematic approach is used to tailor the microgel properties. The size and shape of the microgels is controlled via droplet-based microfluidics. Key requirements for the polymer system, such as absence of cytotoxicity as well as biocompatibility and biodegradability, are accomplished with functionalized poly(ethylene glycol) (PEG), which reacts in a cytocompatible thiol–ene Michael addition. On a mesoscopic level, the microgel stiffness and gelation times are adjusted to obtain high cellular viabilities. The co-encapsulation of living cells provides i) an in vitro platform for the study of cellular metabolic processes which can be applied to bone formation and ii) an in vitro foundation for novel tissue-regenerative therapies. Finally, the degradability of the microgels at physiological conditions caused by hydrolysis-sensitive ester groups in the polymer network is examined.
DDC-Sachgruppe:	540 Chemie 540 Chemistry and allied sciences 570 Biowissenschaften 570 Life sciences
Veröffentlichende Institution:	Johannes Gutenberg-Universität Mainz
Organisationseinheit:	FB 09 Chemie, Pharmazie u. Geowissensch.
Veröffentlichungsort:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-6249
Version:	Published version
Publikationstyp:	Zeitschriftenaufsatz
Nutzungsrechte:	CC BY
Informationen zu den Nutzungsrechten:	https://creativecommons.org/licenses/by/4.0/
Zeitschrift:	Advanced healthcare materials 9 11
Seitenzahl oder Artikelnummer:	1901820
Verlag:	Wiley-VCH
Verlagsort:	Weinheim
Erscheinungsdatum:	2020
ISSN:	2192-2659
URL der Originalveröffentlichung:	https://doi.org/10.1002/adhm.201901820
DOI der Originalveröffentlichung:	10.1002/adhm.201901820
Enthalten in den Sammlungen:	JGU-Publikationen