Authors:	Schwab, Roxana Heller, Martin Pfeifer, Céline Unger, Ronald E. Walenta, Stefan Nezi-Cahn, Sandra Al-Nawas, Bilal Hasenburg, Annette Brenner, Walburgis
Title:	Full-thickness tissue engineered oral mucosa for genitourinary reconstruction : a comparison of different collagen-based biodegradable membranes
Online publication date:	29-Jun-2021
Year of first publication:	2021
Language:	english
Abstract:	Tissue engineering is a method of growing importance regarding clinical application in the genitourinary region. One of the key factors in successfully development of an artificially tissue engineered mucosa equivalent (TEOM) is the optimal choice of the scaffold. Collagen scaffolds are regarded as gold standard in dermal tissue reconstruction. Four distinct collagen scaffolds were evaluated for the ability to support the development of an organotypical tissue architecture. TEOMs were established by seeding cocultures of primary oral epithelial cells and fibroblasts on four distinct collagen membranes. Cell viability was assessed by MTT-assay. The 3D architecture and functionality of the tissue engineered oral mucosa equivalents were evaluated by confocal laser-scanning microscopy and immunostaining. Cell viability was reduced on the TissuFoil E® membrane. A multi-stratified epithelial layer was established on all four materials, however the TEOMs on the Bio-Gide® scaffold showed the best fibroblast differentiation, secretion of tenascin and fibroblast migration into the membrane. The TEOMs generated on Bio-Gide® scaffold exhibited the optimal cellular organization into a cellular 3D network. Thus, the Bio-Gide® scaffold is a suitable matrix for engineering of mucosa substitutes in vitro.
DDC:	610 Medizin 610 Medical sciences
Institution:	Johannes Gutenberg-Universität Mainz
Department:	FB 04 Medizin
Place:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-6131
Version:	Published version
Publication type:	Zeitschriftenaufsatz
License:	CC BY
Information on rights of use:	https://creativecommons.org/licenses/by/4.0/
Journal:	Journal of biomedical materials research : Part B, Applied biomaterials 109 4
Pages or article number:	572 583
Publisher:	Wiley
Publisher place:	Hoboken, NJ
Issue date:	2021
ISSN:	1552-4981
Publisher URL:	https://doi.org/10.1002/jbm.b.34724
Publisher DOI:	10.1002/jbm.b.34724
Appears in collections:	JGU-Publikationen