Autoren:	Zilio, Nicola Ulrich, Helle D.
Titel:	Exploring the SSBreakome : genome-wide mapping of DNA single-strand breaks by next-generation sequencing
Online-Publikationsdatum:	22-Sep-2021
Erscheinungsdatum:	2021
Sprache des Dokuments:	Englisch
Zusammenfassung/Abstract:	Mapping the genome-wide distribution of DNA lesions is key to understanding damage signalling and DNA repair in the context of genome and chromatin structure. Analytical tools based on high-throughput next-generation sequencing have revolutionized our progress with such investigations, and numerous methods are now available for various base lesions and modifications as well as for DNA double-strand breaks. Considering that single-strand breaks are by far the most common type of lesion and arise not only from exposure to exogenous DNA-damaging agents, but also as obligatory intermediates of DNA replication, recombination and repair, it is surprising that our insight into their genome-wide patterns, that is the ‘SSBreakome’, has remained rather obscure until recently, due to a lack of suitable mapping technology. Here we briefly review classical methods for analysing single-strand breaks and discuss and compare in detail a series of recently developed high-resolution approaches for the genome-wide mapping of these lesions, their advantages and limitations and how they have already provided valuable insight into the impact of this type of damage on the genome.
DDC-Sachgruppe:	570 Biowissenschaften 570 Life sciences
Veröffentlichende Institution:	Johannes Gutenberg-Universität Mainz
Organisationseinheit:	FB 10 Biologie
Veröffentlichungsort:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-6097
Version:	Published version
Publikationstyp:	Zeitschriftenaufsatz
Nutzungsrechte:	CC BY-NC
Informationen zu den Nutzungsrechten:	https://creativecommons.org/licenses/by-nc/4.0/
Zeitschrift:	The FEBS journal 288 13
Seitenzahl oder Artikelnummer:	3948 3961
Verlag:	Wiley-Blackwell
Verlagsort:	Oxford u.a.
Erscheinungsdatum:	2021
ISSN:	1742-4658
URL der Originalveröffentlichung:	https://doi.org/10.1111/febs.15568
DOI der Originalveröffentlichung:	10.1111/febs.15568
Enthalten in den Sammlungen:	JGU-Publikationen