Gutenberg Open Science: Exploring the SSBreakome : genome-wide mapping of DNA single-strand breaks by next-generation sequencing

Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-6097

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DC Field	Value	Language
dc.contributor.author	Zilio, Nicola	-
dc.contributor.author	Ulrich, Helle D.	-
dc.date.accessioned	2021-09-22T08:01:44Z	-
dc.date.available	2021-09-22T08:01:44Z	-
dc.date.issued	2021	-
dc.identifier.uri	https://openscience.ub.uni-mainz.de/handle/20.500.12030/6106	-
dc.description.abstract	Mapping the genome-wide distribution of DNA lesions is key to understanding damage signalling and DNA repair in the context of genome and chromatin structure. Analytical tools based on high-throughput next-generation sequencing have revolutionized our progress with such investigations, and numerous methods are now available for various base lesions and modifications as well as for DNA double-strand breaks. Considering that single-strand breaks are by far the most common type of lesion and arise not only from exposure to exogenous DNA-damaging agents, but also as obligatory intermediates of DNA replication, recombination and repair, it is surprising that our insight into their genome-wide patterns, that is the ‘SSBreakome’, has remained rather obscure until recently, due to a lack of suitable mapping technology. Here we briefly review classical methods for analysing single-strand breaks and discuss and compare in detail a series of recently developed high-resolution approaches for the genome-wide mapping of these lesions, their advantages and limitations and how they have already provided valuable insight into the impact of this type of damage on the genome.	en_GB
dc.language.iso	eng	de
dc.rights	CC BY-NC	*
dc.rights.uri	https://creativecommons.org/licenses/by-nc/4.0/	*
dc.subject.ddc	570 Biowissenschaften	de_DE
dc.subject.ddc	570 Life sciences	en_GB
dc.title	Exploring the SSBreakome : genome-wide mapping of DNA single-strand breaks by next-generation sequencing	en_GB
dc.type	Zeitschriftenaufsatz	de
dc.identifier.doi	http://doi.org/10.25358/openscience-6097	-
jgu.type.dinitype	article	en_GB
jgu.type.version	Published version	de
jgu.type.resource	Text	de
jgu.organisation.department	FB 10 Biologie	de
jgu.organisation.number	7970	-
jgu.organisation.name	Johannes Gutenberg-Universität Mainz	-
jgu.rights.accessrights	openAccess	-
jgu.journal.title	The FEBS journal	de
jgu.journal.volume	288	de
jgu.journal.issue	13	de
jgu.pages.start	3948	de
jgu.pages.end	3961	de
jgu.publisher.year	2021	-
jgu.publisher.name	Wiley-Blackwell	de
jgu.publisher.place	Oxford u.a.	de
jgu.publisher.uri	https://doi.org/10.1111/febs.15568	de
jgu.publisher.issn	1742-4658	de
jgu.organisation.place	Mainz	-
jgu.subject.ddccode	570	de
jgu.publisher.doi	10.1111/febs.15568
jgu.organisation.ror	https://ror.org/023b0x485
Appears in collections:	JGU-Publikationen

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