Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-6097
Authors: Zilio, Nicola
Ulrich, Helle D.
Title: Exploring the SSBreakome : genome-wide mapping of DNA single-strand breaks by next-generation sequencing
Online publication date: 22-Sep-2021
Language: english
Abstract: Mapping the genome-wide distribution of DNA lesions is key to understanding damage signalling and DNA repair in the context of genome and chromatin structure. Analytical tools based on high-throughput next-generation sequencing have revolutionized our progress with such investigations, and numerous methods are now available for various base lesions and modifications as well as for DNA double-strand breaks. Considering that single-strand breaks are by far the most common type of lesion and arise not only from exposure to exogenous DNA-damaging agents, but also as obligatory intermediates of DNA replication, recombination and repair, it is surprising that our insight into their genome-wide patterns, that is the ‘SSBreakome’, has remained rather obscure until recently, due to a lack of suitable mapping technology. Here we briefly review classical methods for analysing single-strand breaks and discuss and compare in detail a series of recently developed high-resolution approaches for the genome-wide mapping of these lesions, their advantages and limitations and how they have already provided valuable insight into the impact of this type of damage on the genome.
DDC: 570 Biowissenschaften
570 Life sciences
Institution: Johannes Gutenberg-Universität Mainz
Department: FB 10 Biologie
Place: Mainz
ROR: https://ror.org/023b0x485
DOI: http://doi.org/10.25358/openscience-6097
Version: Published version
Publication type: Zeitschriftenaufsatz
License: CC BY-NC
Information on rights of use: https://creativecommons.org/licenses/by-nc/4.0/
Journal: The FEBS journal
288
13
Pages or article number: 3948
3961
Publisher: Wiley-Blackwell
Publisher place: Oxford u.a.
Issue date: 2021
ISSN: 1742-4658
Publisher URL: https://doi.org/10.1111/febs.15568
Publisher DOI: 10.1111/febs.15568
Appears in collections:JGU-Publikationen

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