Authors:	Zilio, Nicola Ulrich, Helle D.
Title:	Exploring the SSBreakome : genome-wide mapping of DNA single-strand breaks by next-generation sequencing
Online publication date:	22-Sep-2021
Year of first publication:	2021
Language:	english
Abstract:	Mapping the genome-wide distribution of DNA lesions is key to understanding damage signalling and DNA repair in the context of genome and chromatin structure. Analytical tools based on high-throughput next-generation sequencing have revolutionized our progress with such investigations, and numerous methods are now available for various base lesions and modifications as well as for DNA double-strand breaks. Considering that single-strand breaks are by far the most common type of lesion and arise not only from exposure to exogenous DNA-damaging agents, but also as obligatory intermediates of DNA replication, recombination and repair, it is surprising that our insight into their genome-wide patterns, that is the ‘SSBreakome’, has remained rather obscure until recently, due to a lack of suitable mapping technology. Here we briefly review classical methods for analysing single-strand breaks and discuss and compare in detail a series of recently developed high-resolution approaches for the genome-wide mapping of these lesions, their advantages and limitations and how they have already provided valuable insight into the impact of this type of damage on the genome.
DDC:	570 Biowissenschaften 570 Life sciences
Institution:	Johannes Gutenberg-Universität Mainz
Department:	FB 10 Biologie
Place:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-6097
Version:	Published version
Publication type:	Zeitschriftenaufsatz
License:	CC BY-NC
Information on rights of use:	https://creativecommons.org/licenses/by-nc/4.0/
Journal:	The FEBS journal 288 13
Pages or article number:	3948 3961
Publisher:	Wiley-Blackwell
Publisher place:	Oxford u.a.
Issue date:	2021
ISSN:	1742-4658
Publisher URL:	https://doi.org/10.1111/febs.15568
Publisher DOI:	10.1111/febs.15568
Appears in collections:	JGU-Publikationen