Authors:	Matos-Rodrigues, Gabriel E. Grigaravicius, Paulius Lopez, Bernard S. Hofmann, Thomas G. Frappart, Pierre-Olivier Martins, Rodrigo A. P.
Title:	ATRIP protects progenitor cells against DNA damage in vivo
Online publication date:	27-Apr-2021
Year of first publication:	2020
Language:	english
Abstract:	The maintenance of genomic stability during the cell cycle of progenitor cells is essential for the faithful transmission of genetic information. Mutations in genes that ensure genome stability lead to human developmental syndromes. Mutations in Ataxia Telangiectasia and Rad3-related (ATR) or in ATR-interacting protein (ATRIP) lead to Seckel syndrome, which is characterized by developmental malformations and short life expectancy. While the roles of ATR in replicative stress response and chromosomal segregation are well established, it is unknown how ATRIP contributes to maintaining genomic stability in progenitor cells in vivo. Here, we generated the first mouse model to investigate ATRIP function. Conditional inactivation of Atrip in progenitor cells of the CNS and eye led to microcephaly, microphthalmia and postnatal lethality. To understand the mechanisms underlying these malformations, we used lens progenitor cells as a model and found that ATRIP loss promotes replicative stress and TP53-dependent cell death. Trp53 inactivation in Atrip-deficient progenitor cells rescued apoptosis, but increased mitotic DNA damage and mitotic defects. Our findings demonstrate an essential role of ATRIP in preventing DNA damage accumulation during unchallenged replication.
DDC:	610 Medizin 610 Medical sciences
Institution:	Johannes Gutenberg-Universität Mainz
Department:	FB 04 Medizin
Place:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-5843
Version:	Published version
Publication type:	Zeitschriftenaufsatz
License:	CC BY
Information on rights of use:	https://creativecommons.org/licenses/by/4.0/
Journal:	Cell death & disease 11
Pages or article number:	923
Publisher:	Nature Publishing Group
Publisher place:	London
Issue date:	2020
ISSN:	2041-4889
Publisher URL:	https://doi.org/10.1038/s41419-020-03090-9
Publisher DOI:	10.1038/s41419-020-03090-9
Appears in collections:	JGU-Publikationen