Authors:	Hanuscheck, Nicholas Schnatz, Andrea Thalman, Carine Lerch, Steffen Gärtner, Yvonne Domingues, Micaela Bitar, Lynn Nitsch, Robert Zipp, Frauke Vogelaar, Christina F.
Title:	Growth-promoting treatment screening for corticospinal neurons in mouse and man
Online publication date:	3-May-2021
Year of first publication:	2020
Language:	english
Abstract:	Neurons of the central nervous system (CNS) that project long axons into the spinal cord have a poor axon regenerative capacity compared to neurons of the peripheral nervous system. The corticospinal tract (CST) is particularly notorious for its poor regeneration. Because of this, traumatic spinal cord injury (SCI) is a devastating condition that remains as yet uncured. Based on our recent observations that direct neuronal interleukin-4 (IL-4) signaling leads to repair of axonal swellings and beneficial effects in neuroinflammation, we hypothesized that IL-4 acts directly on the CST. Here, we developed a tissue culture model for CST regeneration and found that IL-4 promoted new growth cone formation after axon transection. Most importantly, IL-4 directly increased the regenerative capacity of both murine and human CST axons, which corroborates its regenerative effects in CNS damage. Overall, these findings serve as proof-of-concept that our CST regeneration model is suitable for fast screening of new treatments for SCI.
DDC:	610 Medizin 610 Medical sciences
Institution:	Johannes Gutenberg-Universität Mainz
Department:	FB 04 Medizin
Place:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-5784
Version:	Published version
Publication type:	Zeitschriftenaufsatz
License:	CC BY
Information on rights of use:	https://creativecommons.org/licenses/by/4.0/
Journal:	Cellular and molecular neurobiology 40
Pages or article number:	1327 1338
Publisher:	Springer Science + Business Media B.V
Publisher place:	Dordrecht
Issue date:	2020
ISSN:	1573-6830
Publisher URL:	https://doi.org/10.1007/s10571-020-00820-7
Publisher DOI:	10.1007/s10571-020-00820-7
Appears in collections:	JGU-Publikationen