Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-5643
Authors: Özenver, Nadire
Dawood, Mona
Fleischer, Edmond
Klinger, Anette
Efferth, Thomas
Title: Chemometric and transcriptomic profiling, microtubule disruption and cell death induction by secalonic acid in tumor cells
Online publication date: 9-Feb-2021
Language: english
Abstract: Nature is an indispensable source of new drugs, providing unique bioactive lead structures for drug discovery. In the present study, secalonic acid F (SAF), a naturally occurring ergochrome pigment, was studied for its cytotoxicity against various leukemia and multiple myeloma cells by the resazurin assay. SAF exhibited cytotoxic activity on both leukemia and multiple myeloma cells. Generally, multiple myeloma cells were more sensitive to SAF than leukemia cells. NCI-H929 cells were the most affected cells among the tested panel of multiple myeloma cell lines and were taken for further studies to assess the mode of action of SAF on those cells. Cell cycle analysis revealed that SAF induced S and G2/M arrest in NCI-H929 cells. SAF-associated apoptosis and necrosis resulted in cytotoxicity. SAF further inclined the disassembly of the tubulin network, which may also account for its cytotoxicity. COMPARE and hierarchical cluster analyses of transcriptome-wide expression profiles of the NCI tumor cell line panel identified genes involved in numerous cellular processes (e.g., cell differentiation, cell migration, and other numerous signaling pathways) notably correlated with log10IC50 values for secalonic acid. In conclusion, the present study supports the therapeutic potential of SAF to treat multiple myeloma.
DDC: 570 Biowissenschaften
570 Life sciences
Institution: Johannes Gutenberg-Universität Mainz
Department: FB 09 Chemie, Pharmazie u. Geowissensch.
Place: Mainz
ROR: https://ror.org/023b0x485
DOI: http://doi.org/10.25358/openscience-5643
Version: Published version
Publication type: Zeitschriftenaufsatz
License: CC BY
Information on rights of use: https://creativecommons.org/licenses/by/4.0/
Journal: Molecules
25
14
Pages or article number: 3224
Publisher: MDPI
Publisher place: Basel
Issue date: 2020
ISSN: 1420-3049
Publisher URL: https://doi.org/10.3390/molecules25143224
Publisher DOI: 10.3390/molecules25143224
Appears in collections:JGU-Publikationen

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