Authors:	Düppre, Eva Schneider, Dirk
Title:	The J‐ and G/F‐domains of the major Synechocystis DnaJ protein Sll0897 are sufficient for cell viability but not for heat resistance
Online publication date:	8-Jan-2021
Year of first publication:	2020
Language:	english
Abstract:	Hsp70 proteins and their Hsp40 co‐chaperones are essential components of cellular chaperone networks in both prokaryotes and eukaryotes. Here, we performed a genetic analysis to define the protein domains required for the key functions of the major Hsp40/DnaJ protein Sll0897 of the cyanobacterium Synechocystis sp. PCC6803. The expression of the N‐terminally located J‐ and G/F‐domains is essential and sufficient for the proteins’ fundamental in vivo functions, whereas the presence of the full‐length protein, containing the C‐terminal substrate‐binding domains, is crucial under stress conditions.
DDC:	540 Chemie 540 Chemistry and allied sciences 570 Biowissenschaften 570 Life sciences
Institution:	Johannes Gutenberg-Universität Mainz
Department:	FB 09 Chemie, Pharmazie u. Geowissensch.
Place:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-5543
Version:	Published version
Publication type:	Zeitschriftenaufsatz
License:	CC BY
Information on rights of use:	https://creativecommons.org/licenses/by/4.0/
Journal:	FEBS Open Bio 10 11
Pages or article number:	2343 2349
Publisher:	Wiley
Publisher place:	Hoboken, NJ
Issue date:	2020
ISSN:	2211-5463
Publisher URL:	https://doi.org/10.1002/2211-5463.12980
Publisher DOI:	10.1002/2211-5463.12980
Appears in collections:	JGU-Publikationen