Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-5093
Authors: Lauer, Alina
Wolf, Philipp
Mehler, Dorothea
Götz, Hermann
Rüzgar, Mehmet
Baranowski, Andreas
Henrich, Dirk
Rommens, Pol Maria
Ritz, Ulrike
Title: Biofabrication of SDF-1 functionalized 3D-printed cell-free scaffolds for bone tissue regeneration
Online publication date: 31-Aug-2020
Language: english
Abstract: Large segmental bone defects occurring after trauma, bone tumors, infections or revision surgeries are a challenge for surgeons. The aim of our study was to develop a new biomaterial utilizing simple and cheap 3D-printing techniques. A porous polylactide (PLA) cylinder was printed and functionalized with stromal-derived factor 1 (SDF-1) or bone morphogenetic protein 7 (BMP-7) immobilized in collagen type I. Biomechanical testing proved biomechanical stability and the scaffolds were implanted into a 6 mm critical size defect in rat femur. Bone growth was observed via x-ray and after 8 weeks, bone regeneration was analyzed with µCT and histological staining methods. Development of non-unions was detected in the control group with no implant. Implantation of PLA cylinder alone resulted in a slight but not significant osteoconductive effect, which was more pronounced in the group where the PLA cylinder was loaded with collagen type I. Addition of SDF-1 resulted in an osteoinductive effect, with stronger new bone formation. BMP-7 treatment showed the most distinct effect on bone regeneration. However, histological analyses revealed that newly formed bone in the BMP-7 group displayed a holey structure. Our results confirm the osteoinductive character of this 3D-biofabricated cell-free new biomaterial and raise new options for its application in bone tissue regeneration. Keywords: bone tissue regeneration; 3D printed cell-free scaffold; polylactide; collagen type I; stromal-derived factor 1; in vivo model of critical size defects
DDC: 570 Biowissenschaften
570 Life sciences
610 Medizin
610 Medical sciences
Institution: Johannes Gutenberg-Universität Mainz
Department: FB 04 Medizin
Place: Mainz
DOI: http://doi.org/10.25358/openscience-5093
Version: Published version
Publication type: Zeitschriftenaufsatz
Document type specification: Scientific article
License: CC-BY
Information on rights of use: https://creativecommons.org/licenses/by/4.0/
Journal: International journal of molecular sciences
21
6
Pages or article number: 2175
Publisher: MDPI
Publisher place: Basel
Issue date: 2020
ISSN: 1422-0067
Publisher's URL: https://doi.org/10.3390/ijms21062175
Appears in collections:JGU-Publikationen

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