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Authors: Todorov, Hristo
Kollar, Bettina
Bayer, Franziska
Brandão, Inês
Mann, Amrit
Mohr, Julia
Pontarollo, Giulia
Formes, Henning
Stauber, Roland
Kitter, Jens M.
Endres, Kristina
Watzer, Bernhard
Nockher, Wolfgang Andreas
Sommer, Felix
Gerber, Susanne
Reinhardt, Christoph
Title: α-linolenic acid-rich diet influences microbiota composition and villus morphology of the mouse small intestine
Online publication date: 27-Aug-2020
Language: english
Abstract: α-Linolenic acid (ALA) is well-known for its anti-inflammatory activity. In contrast, the influence of an ALA-rich diet on intestinal microbiota composition and its impact on small intestine morphology are not fully understood. In the current study, we kept adult C57BL/6J mice for 4 weeks on an ALA-rich or control diet. Characterization of the microbial composition of the small intestine revealed that the ALA diet was associated with an enrichment in Prevotella and Parabacteroides. In contrast, taxa belonging to the Firmicutes phylum, including Lactobacillus, Clostridium cluster XIVa, Lachnospiraceae and Streptococcus, had significantly lower abundance compared to control diet. Metagenome prediction indicated an enrichment in functional pathways such as bacterial secretion system in the ALA group, whereas the two-component system and ALA metabolism pathways were downregulated. We also observed increased levels of ALA and its metabolites eicosapentanoic and docosahexanoic acid, but reduced levels of arachidonic acid in the intestinal tissue of ALA-fed mice. Furthermore, intestinal morphology in the ALA group was characterized by elongated villus structures with increased counts of epithelial cells and reduced epithelial proliferation rate. Interestingly, the ALA diet reduced relative goblet and Paneth cell counts. Of note, high-fat Western-type diet feeding resulted in a comparable adaptation of the small intestine. Collectively, our study demonstrates the impact of ALA on the gut microbiome and reveals the nutritional regulation of gut morphology. Keywords: α-linolenic acid; microbiota; epithelial renewal; goblet cells; paneth cells; villus morphology
DDC: 610 Medizin
610 Medical sciences
Institution: Johannes Gutenberg-Universität Mainz
Department: FB 04 Medizin
Place: Mainz
Version: Published version
Publication type: Zeitschriftenaufsatz
Document type specification: Scientific article
License: CC-BY
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Journal: Nutrients
Pages or article number: 732
Publisher: MDPI
Publisher place: Basel
Issue date: 2020
ISSN: 2072-6643
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Appears in collections:JGU-Publikationen

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