Authors:	Tueting, Laura
Title:	Molecular Dynamics Investigated by Plasmon Rulers
Online publication date:	1-May-2019
Year of first publication:	2019
Language:	english
Abstract:	Plasmon rulers consisting of two connected gold nanoparticles respond to molecular distance changes with a spectral shift in resonance wavelength accompanied with a total intensity change. Here, I introduce the use of plasmon rulers as a single-molecule technique for the elucidation of conformational and stiffness changes in single polymers and proteins. The equilibrium fluctuations of a molecule’s extension are governed by intrinsic molecular properties. Monitoring plasmon ruler trajectories, I obtain the stiffness and extension of polyethylene glycol in varying salt concentrations of different cations. I observe a transition above about 1.2mM K+ ion concentration which is specific to potassium ions and does not occur in the presence of sodium ions. Further I investigate the conformational cycle of the molecular machine Hsp90. With the help of the plasmon ruler’s unprecedented observation bandwidth, I detect a third state inaccessible with other methods. This new state is characterized by dwell times in the order of dozens of seconds which are rare events only occurring in 1% of the cases. Further the influence of nucleotides on the protein is studied leading to a clear shift of the equilibrium.
DDC:	540 Chemie 540 Chemistry and allied sciences
Institution:	Johannes Gutenberg-Universität Mainz
Department:	FB 09 Chemie, Pharmazie u. Geowissensch.
Place:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-4824
URN:	urn:nbn:de:hebis:77-diss-1000027453
Version:	Original work
Publication type:	Dissertation
License:	In Copyright
Information on rights of use:	https://rightsstatements.org/vocab/InC/1.0/
Extent:	157 Seiten
Appears in collections:	JGU-Publikationen