Authors:	Manicam, Caroline Perumal, Natarajan Wasielica-Poslednik, Joanna Ngongkole, Yong Cajetan Tschäbunin, Alexandra Sievers, Marcel Lisch, Walter Pfeiffer, Norbert Grus, Franz-Hermann Gericke, Adrian
Title:	Proteomics unravels the regulatory mechanisms in human tears following acute renouncement of contact lens use : a comparison between hard and soft lenses
Online publication date:	19-Dec-2018
Year of first publication:	2018
Language:	english
Abstract:	Contact lenses (CLs) provide a superior alternative to spectacles. Although beneficial, the global burden of ocular dysfunctions attributed to regular use of CLs remains a topic of much challenge in ophthalmic research owing to debilitating clinical repercussions on the ocular surface, which are often manifested as breach in tear film integrity. This study elucidated the intricate tear proteome changes attributed to the use of different CLs (hard and soft) and unravelled, for the first time, the restorative mechanisms of several protein clusters following acute renouncement of CL use employing the label-free mass spectrometry-based quantitative proteomics approach. The expression patterns of certain proteins clusters were specific to the use of a particular lens type and a large majority of these actively regulates cell death and survival and, modulates cellular movement on the ocular surface. Noteworthy, CL use also evoked a significant upregulation of glycolytic enzymes associated with hypoxia and corresponding cognate metabolic pathways, particularly glucose metabolism and FXR/RXR pathways. Importantly, the assessment of CL renouncement unravelled the restorative properties of several clusters of proteins involved mainly in organismal injury and abnormalities and, cellular function and maintenance. These proteins play key roles in restoring tear homeostasis and wound-healing mechanisms post-CL use-elicited injury.
DDC:	610 Medizin 610 Medical sciences
Institution:	Johannes Gutenberg-Universität Mainz
Department:	FB 04 Medizin
Place:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-253
Version:	Published version
Publication type:	Zeitschriftenaufsatz
License:	CC BY
Information on rights of use:	https://creativecommons.org/licenses/by/4.0/
Journal:	Scientific reports 8
Pages or article number:	Art. 11526
Publisher:	Macmillan Publishers Limited, part of Springer Nature
Publisher place:	London
Issue date:	2018
ISSN:	2045-2322
Publisher URL:	http://dx.doi.org/10.1038/s41598-018-30032-5
Publisher DOI:	10.1038/s41598-018-30032-5
Appears in collections:	JGU-Publikationen