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Authors: Mikuličić, Snježana
Finke, Jérôme
Boukhallouk, Fatima
Wüstenhagen, Elena
Sons, Dominik
Homsi, Yahya
Reiss, Karina
Lang, Thorsten
Florin, Luise
Title: ADAM17-dependent signaling is required for oncogenic human papillomavirus entry platform assembly
Online publication date: 8-Jul-2019
Language: english
Abstract: Oncogenic human papillomaviruses (HPV) are small DNA viruses that infect keratinocytes. After HPV binding to cell surface receptors, a cascade of molecular interactions mediates the infectious cellular internalization of virus particles. Aside from the virus itself, important molecular players involved in virus entry include the tetraspanin CD151 and the epidermal growth factor receptor (EGFR). To date, it is unknown how these components are coordinated in space and time. Here, we studied plasma membrane dynamics of CD151 and EGFR and the HPV16 capsid during the early phase of infection. We find that the proteinase ADAM17 activates the extracellular signal-regulated kinases (ERK1/2) pathway by the shedding of growth factors which triggers the formation of an endocytic entry platform. Infectious endocytic entry platforms carrying virus particles consist of two-fold larger CD151 domains containing the EGFR. Our finding clearly dissects initial virus binding from ADAM17-dependent assembly of a HPV/CD151/EGFR entry platform.
DDC: 610 Medizin
610 Medical sciences
Institution: Johannes Gutenberg-Universität Mainz
Department: FB 04 Medizin
Place: Mainz
Version: Published version
Publication type: Zeitschriftenaufsatz
License: CC-BY
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Journal: eLife
Pages or article number: e44345
Publisher: eLife Sciences Publications
Publisher place: Cambridge
Issue date: 2019
ISSN: 2050-084X
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Appears in collections:JGU-Publikationen

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