Protocol for the collection and analysis of the different immune cell subsets in the murine intestinal lamina propria

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dc.contributor.authorShanmugavadivu, Arthi
dc.contributor.authorCarter, Katlynn
dc.contributor.authorPoursadegh Zonouzi, Aysan
dc.contributor.authorWaisman, Ari
dc.contributor.authorRegen, Tommy
dc.date.accessioned2024-12-19T16:17:18Z
dc.date.available2024-12-19T16:17:18Z
dc.date.issued2024
dc.description.abstractThe intestinal lamina propria (LP) is a leukocyte-rich cornerstone of the immune system owing to its vital role in immune surveillance and barrier defense against external pathogens. Here, we present a protocol for isolating and analyzing immune cell subsets from the mouse intestinal LP for further downstream applications. Starting from tissue collection and cleaning, epithelium removal, and enzymatic digestion to collection of single cells, we explain each step in detail to maximize the yield of immune cells from the intestinal LP.en_GB
dc.identifier.doihttp://doi.org/10.25358/openscience-11166
dc.identifier.urihttps://openscience.ub.uni-mainz.de/handle/20.500.12030/11187
dc.language.isoeng
dc.rightsCC-BY-NC-4.0
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.subject.ddc610 Medizinde_DE
dc.subject.ddc610 Medical sciencesen_GB
dc.titleProtocol for the collection and analysis of the different immune cell subsets in the murine intestinal lamina propriaen_GB
dc.typeZeitschriftenaufsatzde_DE
jgu.apc.netprice1139,00
jgu.apc.price1218,73
jgu.apc.taxrate7
jgu.apc.transformationcontractElsevier
jgu.dfg.year2024
jgu.journal.issue3
jgu.journal.titleSTAR Protocols
jgu.journal.volume5
jgu.nationalcurrency.eur1139,00
jgu.organisation.departmentFB 04 Medizinde_DE
jgu.organisation.nameJohannes Gutenberg-Universität Mainzde_DE
jgu.organisation.number2700
jgu.organisation.placeMainz
jgu.organisation.rorhttps://ror.org/023b0x485
jgu.pages.alternative103154
jgu.publisher.doi10.1016/j.xpro.2024.103154
jgu.publisher.issn2666-1667
jgu.publisher.nameCell Press
jgu.publisher.placeCambridge, MA
jgu.publisher.year2024
jgu.rights.accessrightsopenAccessen_GB
jgu.subject.ddccode610
jgu.subject.dfgLebenswissenschaftende_DE
jgu.type.dinitypeArticleen_GB
jgu.type.resourceTexten_GB
jgu.type.versionPublished versionen_GB

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