Towards optimized tissue regeneration : a new 3D printable bioink of alginate/cellulose hydrogel loaded with thrombocyte concentrate
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Abstract
Introduction: Autologous platelet concentrate (APC) are pro-angiogenic and can
promote wound healing and tissue repair, also in combination with other
biomaterials. However, challenging defect situations remain demanding. 3D
bioprinting of an APC based bioink encapsulated in a hydrogel could overcome
this limitation with enhanced physio-mechanical interface, growth factor retention/
secretion and defect-personalized shape to ultimately enhance regeneration.
Methods: This study used extrusion-based bioprinting to create a novel bioink of
alginate/cellulose hydrogel loaded with thrombocyte concentrate. Chemicophysical testing exhibited an amorphous structure characterized by high shape
fidelity. Cytotoxicity assay and incubation of human osteogenic sarcoma cells
(SaOs2) exposed excellent biocompatibility. enzyme-linked immunosorbent
assay analysis confirmed pro-angiogenic growth factor release of the printed
constructs, and co-incubation with HUVECS displayed proper cell viability and
proliferation. Chorioallantoic membrane (CAM) assay explored the pro angiogenic potential of the prints in vivo. Detailed proteome and secretome
analysis revealed a substantial amount and homologous presence of pro angiogenic proteins in the 3D construct.
Results: This study demonstrated a 3D bioprinting approach to fabricate a novel
bioink of alginate/cellulose hydrogel loaded with thrombocyte concentrate with
high shape fidelity, biocompatibility, and substantial pro-angiogenic properties.
Conclusion: This approach may be suitable for challenging physiological and
anatomical defect situations when translated into clinical use.
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Frontiers in Bioengineering and Biotechnology, 12, Frontiers Media, Lausanne, 2024, https://doi.org/10.3389/fbioe.2024.1363380