Protocol for the differentiation of induced pluripotent stem cells into retinal pigment epithelial cells
| dc.contributor.author | Zorin, Mark | |
| dc.contributor.author | Ewerling-Hähnel, Rike | |
| dc.contributor.author | Nagel-Wolfrum, Kerstin | |
| dc.date.accessioned | 2026-02-25T14:29:03Z | |
| dc.date.issued | 2025 | |
| dc.description.abstract | Induced pluripotent stem cells (iPSCs) are emerging as a valuable system for modeling tissues and organs. Here, we describe a highly scalable protocol for the differentiation of iPSCs into retinal pigment epithelium (RPE), including a new step that makes it easier for researchers to obtain a high-purity culture. We also describe a cryopreservation technique for RPE progenitor cells to enable their storage. The use of cryopreserved cells allows a subsequent reduction in differentiation time compared to the full protocol. | en |
| dc.identifier.doi | https://doi.org/10.25358/openscience-14528 | |
| dc.identifier.uri | https://openscience.ub.uni-mainz.de/handle/20.500.12030/14549 | |
| dc.language.iso | eng | |
| dc.rights | CC-BY-4.0 | |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
| dc.subject.ddc | 570 Biowissenschaften | de |
| dc.subject.ddc | 570 Life sciences | en |
| dc.title | Protocol for the differentiation of induced pluripotent stem cells into retinal pigment epithelial cells | en |
| dc.type | Zeitschriftenaufsatz | |
| jgu.identifier.uuid | 269dbd55-e530-44c5-96d0-9024b40b24e9 | |
| jgu.journal.issue | 4 | |
| jgu.journal.title | STAR Protocols | |
| jgu.journal.volume | 6 | |
| jgu.organisation.department | FB 10 Biologie | |
| jgu.organisation.name | Johannes Gutenberg-Universität Mainz | |
| jgu.organisation.number | 7970 | |
| jgu.organisation.place | Mainz | |
| jgu.organisation.ror | https://ror.org/023b0x485 | |
| jgu.pages.alternative | 104263 | |
| jgu.publisher.doi | 10.1016/j.xpro.2025.104263 | |
| jgu.publisher.eissn | 2666-1667 | |
| jgu.publisher.name | Cell Press | |
| jgu.publisher.place | Cambridge, MA | |
| jgu.publisher.year | 2025 | |
| jgu.rights.accessrights | openAccess | |
| jgu.subject.ddccode | 570 | |
| jgu.subject.dfg | Lebenswissenschaften | |
| jgu.type.dinitype | Article | en_GB |
| jgu.type.resource | Text | |
| jgu.type.version | Published version |