Please use this identifier to cite or link to this item:
http://doi.org/10.25358/openscience-9604
Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Zahnreich, Sebastian | - |
dc.contributor.author | El Guerzyfy, Soumia | - |
dc.contributor.author | Kaufmann, Justus | - |
dc.contributor.author | Schmidberger, Heinz | - |
dc.date.accessioned | 2023-11-21T08:41:28Z | - |
dc.date.available | 2023-11-21T08:41:28Z | - |
dc.date.issued | 2023 | - |
dc.identifier.uri | https://openscience.ub.uni-mainz.de/handle/20.500.12030/9622 | - |
dc.description.abstract | Locally advanced head and neck squamous cell carcinomas (HNSCC) are often refractory to platinum-based radiochemotherapy and new immuno-oncological strategies. To stimulate immunogenic antitumor responses in HNSCC patients, we investigated the cGAS/STING/IFN-1 signaling pathway after genotoxic treatments and concomitant abrogation of the DNA damage response (DDR). For this purpose, FaDu and UM-SCC1 cells were exposed to X-rays or cisplatin and treated with an ATR or Chk1 inhibitor, or by Fanconi anemia gene A knockout (FANCA ko). We assessed clonogenic survival, cell cycle regulation, micronuclei, free cytosolic double-stranded DNA, and the protein expression and activity of the cGAS/STING/IFN-1 pathway and related players. Cell survival, regulation of G2/M arrest, and formation of rupture-prone cGAS-positive micronuclei after genotoxic treatments were most affected by ATR inhibition and FANCA ko. In UM-SCC-1 cells only, 8 Gy X-rays promoted IFN-1 expression unaltered by abrogation of the DDR or concomitant increased TREX1 expression. At a higher dose of 20 Gy, this effect was observed only for concurrent Chk1- or ATR-inhibition. FANCA ko or cisplatin treatment was ineffective in this regard. Our observations open new perspectives for the enhancement of cGAS/STING/IFN-1-mediated antitumor immune response in HNSCC by hypofractionated or stereotactic radiotherapy concepts in multimodal settings with immuno-oncological strategies. | en_GB |
dc.language.iso | eng | de |
dc.rights | CC BY | * |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | * |
dc.subject.ddc | 570 Biowissenschaften | de_DE |
dc.subject.ddc | 570 Life sciences | en_GB |
dc.subject.ddc | 610 Medizin | de_DE |
dc.subject.ddc | 610 Medical sciences | en_GB |
dc.title | The cGAS/STING/IFN-1 response in squamous head and neck cancer cells after genotoxic challenges and abrogation of the ATR-Chk1 and Fanconi anemia axis | en_GB |
dc.type | Zeitschriftenaufsatz | de |
dc.identifier.doi | http://doi.org/10.25358/openscience-9604 | - |
jgu.type.contenttype | Scientific article | de |
jgu.type.dinitype | article | en_GB |
jgu.type.version | Published version | de |
jgu.type.resource | Text | de |
jgu.organisation.department | FB 04 Medizin | de |
jgu.organisation.number | 2700 | - |
jgu.organisation.name | Johannes Gutenberg-Universität Mainz | - |
jgu.rights.accessrights | openAccess | - |
jgu.journal.title | International journal of molecular sciences | de |
jgu.journal.volume | 24 | de |
jgu.journal.issue | 19 | de |
jgu.pages.alternative | 14900 | de |
jgu.publisher.year | 2023 | - |
jgu.publisher.name | Molecular Diversity Preservation International | de |
jgu.publisher.place | Basel | de |
jgu.publisher.issn | 1422-0067 | de |
jgu.organisation.place | Mainz | - |
jgu.subject.ddccode | 570 | de |
jgu.subject.ddccode | 610 | de |
jgu.publisher.doi | 10.3390/ijms241914900 | de |
jgu.organisation.ror | https://ror.org/023b0x485 | - |
jgu.subject.dfg | Naturwissenschaften | de |
Appears in collections: | DFG-491381577-G |
Files in This Item:
File | Description | Size | Format | ||
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the_cgasstingifn1_response_in-20231012103021973.pdf | 2.26 MB | Adobe PDF | View/Open |