Autoren:	Knoll, Rebecca L. Jarquín‑Díaz, Víctor Hugo Klopp, Jonas Kemper, Alissa Hilbert, Katja Hillen, Barlo Pfirrmann, Daniel Simon, Perikles Bähner, Viola Nitsche, Oliver Gehring, Stephan Markó, Lajos Forslund, Sofia K. Poplawska, Krystyna
Titel:	Resilience and stability of the CF- intestinal and respiratory microbiome during nutritional and exercise intervention
Online-Publikationsdatum:	25-Aug-2023
Erscheinungsdatum:	2023
Sprache des Dokuments:	Englisch
Zusammenfassung/Abstract:	Background Impaired respiratory and intestinal microbiome composition is linked to cystic fibrosis lung disease severity. In people with cystic fibrosis (pwCF), regular exercise is recommended to delay disease progression and preserve a stable lung function. An optimal nutritional status is vital for best clinical outcomes. Our study investigated whether regular and monitored exercise and nutritional support promotes CF microbiome health. Methods A personalized nutrition and exercise program promoted nutritional intake and physical fitness in 18 pwCF for 12 months. Throughout the study, patients performed strength and endurance training monitored by a sports scientist via an internet platform. After three months, food supplementation with Lactobacillus rhamnosus LGG was introduced. Nutritional status and physical fitness were assessed before the study started, after three and nine months. Sputum and stool were collected, and microbial composition was analyzed by 16S rRNA gene sequencing. Results Sputum and stool microbiome composition remained stable and highly specific to each patient during the study period. Disease-associated pathogens dominated sputum composition. Lung disease severity and recent antibiotic treatment had the highest impact on taxonomic composition in stool and sputum microbiome. Strikingly, the long-term antibiotic treatment burden had only a minor influence. Conclusion Despite the exercise and nutritional intervention, respiratory and intestinal microbiomes proved to be resilient. Dominant pathogens drove the composition and functionality of the microbiome. Further studies are required to understand which therapy could destabilize the dominant disease-associated microbial composition of pwCF.
DDC-Sachgruppe:	610 Medizin 610 Medical sciences
Veröffentlichende Institution:	Johannes Gutenberg-Universität Mainz
Organisationseinheit:	FB 04 Medizin
Veröffentlichungsort:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-9438
Version:	Published version
Publikationstyp:	Zeitschriftenaufsatz
Weitere Angaben zur Dokumentart:	Scientific article
Nutzungsrechte:	CC BY
Informationen zu den Nutzungsrechten:	https://creativecommons.org/licenses/by/4.0/
Zeitschrift:	BMC microbiology 23
Seitenzahl oder Artikelnummer:	44
Verlag:	BioMed Central
Verlagsort:	London
Erscheinungsdatum:	2023
ISSN:	1471-2180
URL der Originalveröffentlichung:	https://doi.org/10.1186/s12866-023-02788-y
DOI der Originalveröffentlichung:	10.1186/s12866-023-02788-y
Enthalten in den Sammlungen:	DFG-491381577-G