Autoren:	Bessler, Larissa Vogt, Lea-Marie Lander, Marc Dal Magro, Christina Keller, Patrick Kühlborn, Jonas Kampf, Christopher J. Opatz, Till Helm, Mark
Titel:	A new bacterial adenosine-derived nucleoside as an example of RNA modification damage
Online-Publikationsdatum:	27-Apr-2023
Erscheinungsdatum:	2023
Sprache des Dokuments:	Englisch
Zusammenfassung/Abstract:	The fields of RNA modification and RNA damage both exhibit a plethora of non-canonical nucleo side structures. While RNA modifications have evolved to improve RNA function, the term RNA damage implies detrimental effects. Based on stable isotope labelling and mass spectrometry, we report the identi fication and characterisation of 2-methylthio-1,N6-ethe noadenosine (ms2 ɛA), which is related to 1,N6-etheno adenine, a lesion resulting from exposure of nucleic acids to alkylating chemicals in vivo. In contrast, a sophisticated isoprene labelling scheme revealed that ms2 ɛA biogenesis involves cleavage of a prenyl moiety in the known transfer RNA (tRNA) modification 2-meth ylthio-N6-isopentenyladenosine (ms2 i 6 A). The relative abundance of ms2 ɛA in tRNAs from translating ribo somes suggests reduced function in comparison to its parent RNA modification, establishing the nature of the new structure in a newly perceived overlap of the two previously separate fields, namely an RNA modification damage.
DDC-Sachgruppe:	540 Chemie 540 Chemistry and allied sciences
Veröffentlichende Institution:	Johannes Gutenberg-Universität Mainz
Organisationseinheit:	FB 09 Chemie, Pharmazie u. Geowissensch.
Veröffentlichungsort:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-9052
Version:	Published version
Publikationstyp:	Zeitschriftenaufsatz
Nutzungsrechte:	CC BY
Informationen zu den Nutzungsrechten:	https://creativecommons.org/licenses/by/4.0/
Zeitschrift:	Angewandte Chemie : International edition 62 11
Seitenzahl oder Artikelnummer:	e202217128
Verlag:	Wiley-VCH
Verlagsort:	Weinheim
Erscheinungsdatum:	2023
ISSN:	1521-3773
DOI der Originalveröffentlichung:	10.1002/anie.202217128
Beziehungen:	is version of 10.25358/openscience-10177
Enthalten in den Sammlungen:	DFG-491381577-H