Authors:	Bessler, Larissa Vogt, Lea-Marie Lander, Marc Dal Magro, Christina Keller, Patrick Kühlborn, Jonas Kampf, Christopher J. Opatz, Till Helm, Mark
Title:	A new bacterial adenosine-derived nucleoside as an example of RNA modification damage
Online publication date:	27-Apr-2023
Year of first publication:	2023
Language:	english
Abstract:	The fields of RNA modification and RNA damage both exhibit a plethora of non-canonical nucleo side structures. While RNA modifications have evolved to improve RNA function, the term RNA damage implies detrimental effects. Based on stable isotope labelling and mass spectrometry, we report the identi fication and characterisation of 2-methylthio-1,N6-ethe noadenosine (ms2 ɛA), which is related to 1,N6-etheno adenine, a lesion resulting from exposure of nucleic acids to alkylating chemicals in vivo. In contrast, a sophisticated isoprene labelling scheme revealed that ms2 ɛA biogenesis involves cleavage of a prenyl moiety in the known transfer RNA (tRNA) modification 2-meth ylthio-N6-isopentenyladenosine (ms2 i 6 A). The relative abundance of ms2 ɛA in tRNAs from translating ribo somes suggests reduced function in comparison to its parent RNA modification, establishing the nature of the new structure in a newly perceived overlap of the two previously separate fields, namely an RNA modification damage.
DDC:	540 Chemie 540 Chemistry and allied sciences
Institution:	Johannes Gutenberg-Universität Mainz
Department:	FB 09 Chemie, Pharmazie u. Geowissensch.
Place:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-9052
Version:	Published version
Publication type:	Zeitschriftenaufsatz
License:	CC BY
Information on rights of use:	https://creativecommons.org/licenses/by/4.0/
Journal:	Angewandte Chemie : International edition 62 11
Pages or article number:	e202217128
Publisher:	Wiley-VCH
Publisher place:	Weinheim
Issue date:	2023
ISSN:	1521-3773
Publisher DOI:	10.1002/anie.202217128
Relations:	is version of 10.25358/openscience-10177
Appears in collections:	DFG-491381577-H