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http://doi.org/10.25358/openscience-9019
Autoren: | Zimmermann, Robert A. Fischer, Tim R. Schwickert, Marvin Nidoieva, Zarina Schirmeister, Tanja Kersten, Christian |
Titel: | Chemical space virtual screening against hard-to-drug RNA methyltransferases DNMT2 and NSUN6 |
Online-Publikationsdatum: | 18-Apr-2023 |
Erscheinungsdatum: | 2023 |
Sprache des Dokuments: | Englisch |
Zusammenfassung/Abstract: | Targeting RNA methyltransferases with small molecules as inhibitors or tool compounds is an emerging field of interest in epitranscriptomics and medicinal chemistry. For two challenging RNA methyltransferases that introduce the 5-methylcytosine (m5C) modification in different tRNAs, namely DNMT2 and NSUN6, an ultra-large commercially available chemical space was virtually screened by physicochemical property filtering, molecular docking, and clustering to identify new ligands for those enzymes. Novel chemotypes binding to DNMT2 and NSUN6 with affinities down to KD,app = 37 μM and KD,app = 12 μM, respectively, were identified using a microscale thermophoresis (MST) binding assay. These compounds represent the first molecules with a distinct structure from the cofactor SAM and have the potential to be developed into activity-based probes for these enzymes. Additionally, the challenges and strategies of chemical space docking screens with special emphasis on library focusing and diversification are discussed. |
DDC-Sachgruppe: | 540 Chemie 540 Chemistry and allied sciences |
Veröffentlichende Institution: | Johannes Gutenberg-Universität Mainz |
Organisationseinheit: | FB 09 Chemie, Pharmazie u. Geowissensch. |
Veröffentlichungsort: | Mainz |
ROR: | https://ror.org/023b0x485 |
DOI: | http://doi.org/10.25358/openscience-9019 |
Version: | Published version |
Publikationstyp: | Zeitschriftenaufsatz |
Weitere Angaben zur Dokumentart: | Scientific article |
Nutzungsrechte: | CC BY |
Informationen zu den Nutzungsrechten: | https://creativecommons.org/licenses/by/4.0/ |
Zeitschrift: | International Journal of Molecular Sciences 24 7 |
Seitenzahl oder Artikelnummer: | 6109 |
Verlag: | MDPI |
Verlagsort: | Basel |
Erscheinungsdatum: | 2023 |
ISSN: | 1422-0067 |
DOI der Originalveröffentlichung: | 10.3390/ijms24076109 |
Enthalten in den Sammlungen: | DFG-491381577-G |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | ||
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chemical_space_virtual_screen-20230418075719562.pdf | 13.7 MB | Adobe PDF | Öffnen/Anzeigen |