Please use this identifier to cite or link to this item:
Authors: Liu, Wei
Samanta, Avik
Deng, Jie
Akintayo, Cecilia Oluwadunsin
Walther, Andreas
Title: Mechanistic insights into the phase separation behavior and pathway-directed information exchange in all-DNA droplets
Online publication date: 9-Feb-2023
Year of first publication: 2022
Language: english
Abstract: Liquid-liquid phase separation provides a versatile approach to fabricating cell-mimicking coacervates. Recently, it was discovered that phase separation of single-stranded DNA (ssDNA) allows for forming protocells and microgels in multicomponent systems. However, the mechanism of the ssDNA phase separation is not comprehensively understood. Here, we present mechanistic insights into the metal-dependent phase separation of ssDNA and leverage this understanding for a straightforward formation of all-DNA droplets. Two phase separation temperatures are found that correspond to the formation of primary nuclei and a growth process. Ca2+ allows for irreversible, whereas Mg2+ leads to reversible phase separation. Capitalizing on these differences makes it possible to control the information transfer of one-component DNA droplets and two-component core–shell protocells. This study introduces new kinetic traps of phase separating ssDNA that lead to new phenomena in cell-mimicking systems.
DDC: 540 Chemie
540 Chemistry and allied sciences
Institution: Johannes Gutenberg-Universität Mainz
Department: FB 09 Chemie, Pharmazie u. Geowissensch.
Place: Mainz
Version: Published version
Publication type: Zeitschriftenaufsatz
License: CC BY-NC
Information on rights of use:
Journal: Angewandte Chemie : International edition
Pages or article number: e202208951
Publisher: Wiley-VCH
Publisher place: Weinheim
Issue date: 2022
ISSN: 1521-3773
Publisher DOI: 10.1002/anie.202208951
Appears in collections:DFG-491381577-H

Files in This Item:
  File Description SizeFormat
echanistic_insights_into_the_-20230209111201559.pdf4.34 MBAdobe PDFView/Open