Authors:	Groeer, Saskia Garni, Martina Samanta, Avik Walther, Andreas
Title:	Insertion of 3D DNA origami nanopores into block copolymer vesicles
Online publication date:	2-Feb-2023
Year of first publication:	2022
Language:	english
Abstract:	Block copolymer-based polymersomes are important building blocks for the bottom-up design of protocells and are considered advantageous over liposomes due to their higher mechanical stability and chemical versatility. Endowing both types of vesicles with capabilities for transmembrane transport is important for creating nanoreactor functionality and has been achieved by insertion of protein nanopores, even into comparably thick polymersome membranes. Still, the design space for protein nanopores is limited and higher flexibility might be accessible by de novo design of DNA nanopores, which have thus far been limited largely to liposome systems. Here, we introduce the successful insertion of two different 3D DNA origami nanopores into PMOXA-b-PDMS-b-PMOXA polymersomes, and confirm pore formation by dye influx studies and microscopy. This research thus opens the further design space of this versatile class of large DNA origami nanopores for polymersome-based functional protocells.
DDC:	540 Chemie 540 Chemistry and allied sciences
Institution:	Johannes Gutenberg-Universität Mainz
Department:	FB 09 Chemie, Pharmazie u. Geowissensch.
Place:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-8715
Version:	Published version
Publication type:	Zeitschriftenaufsatz
Document type specification:	Scientific article
License:	CC BY-NC-ND
Information on rights of use:	https://creativecommons.org/licenses/by-nc-nd/4.0/
Journal:	ChemSystemsChem 4 6
Pages or article number:	202200009
Publisher:	John Wiley & Sons, Ltd
Publisher place:	Weinheim
Issue date:	2022
ISSN:	25704206
Publisher DOI:	10.1002/syst.202200009
Appears in collections:	DFG-491381577-H