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http://doi.org/10.25358/openscience-8634
Autoren: | Schildknecht, Stefan Kriegsheim, Alex von Vujacic-Mirski, Ksenija Di Lisa, Fabio Ullrich, Volker Daiber, Andreas |
Titel: | Recovery of reduced thiol groups by superoxide-mediated denitrosation of nitrosothiols |
Online-Publikationsdatum: | 25-Jan-2023 |
Erscheinungsdatum: | 2022 |
Sprache des Dokuments: | Englisch |
Zusammenfassung/Abstract: | Nitrosation of critical thiols has been elaborated as reversible posttranslational modification with regulatory function in multiple disorders. Reversibility of S-nitrosation is generally associated with enzyme-mediated one-electron reductions, catalyzed by the thioredoxin system, or by nitrosoglutathione reductase. In the present study, we confirm previous evidence for a non-enzymatic de-nitrosation of nitrosoglutathione (GSNO) by superoxide. The interaction leads to the release of nitric oxide that subsequently interacts with a second molecule of superoxide (O2•−) to form peroxynitrite. Despite the formation of peroxynitrite, approximately 40–70% of GSNO yielded reduced glutathione (GSH), depending on the applied analytical assay. The concept of O2•− dependent denitrosation was then applied to S-nitrosated enzymes. S-nitrosation of isocitrate dehydrogenase (ICDH; NADP+-dependent) was accompanied by an inhibition of the enzyme and could be reversed by dithiothreitol. Treatment of nitrosated ICDH with O2•− indicated ca. 50% recovery of enzyme activity. Remaining inhibition was largely consequence of oxidative modifications evoked either by O2•− or by peroxynitrite. Recovery of activity in S-nitrosated enzymes by O2•− appears relevant only for selected examples. In contrast, recovery of reduced glutathione from the interaction of GSNO with O2•− could represent a mechanism to regain reducing equivalents in situations of excess O2•− formation, e.g. in the reperfusion phase after ischemia. |
DDC-Sachgruppe: | 610 Medizin 610 Medical sciences |
Veröffentlichende Institution: | Johannes Gutenberg-Universität Mainz |
Organisationseinheit: | FB 04 Medizin |
Veröffentlichungsort: | Mainz |
ROR: | https://ror.org/023b0x485 |
DOI: | http://doi.org/10.25358/openscience-8634 |
Version: | Published version |
Publikationstyp: | Zeitschriftenaufsatz |
Weitere Angaben zur Dokumentart: | Scientific article |
Nutzungsrechte: | CC BY-NC-ND |
Informationen zu den Nutzungsrechten: | https://creativecommons.org/licenses/by-nc-nd/4.0/ |
Zeitschrift: | Redox Biology 56 |
Seitenzahl oder Artikelnummer: | 102439 |
Verlag: | Elsevier |
Verlagsort: | Amsterdam |
Erscheinungsdatum: | 2022 |
ISSN: | 2213-2317 |
DOI der Originalveröffentlichung: | 10.1016/j.redox.2022.102439 |
Enthalten in den Sammlungen: | DFG-491381577-G |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | ||
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recovery_of_reduced_thiol_gro-20230124112147523.pdf | 8.44 MB | Adobe PDF | Öffnen/Anzeigen |