Bitte benutzen Sie diese Kennung, um auf die Ressource zu verweisen:
http://doi.org/10.25358/openscience-8586
Autoren: | Hetsch, Florian Wang, Danni Chen, Xufeng Zhang, Jiong Aslam, Muhammad Kegel, Marcel Tonner, Henrik Grus, Franz Engelhardt, Jakob von |
Titel: | CKAMP44 controls synaptic function and strength of relay neurons during early development of the dorsal lateral geniculate nucleus |
Online-Publikationsdatum: | 19-Jan-2023 |
Erscheinungsdatum: | 2022 |
Sprache des Dokuments: | Englisch |
Zusammenfassung/Abstract: | Relay neurons of the dorsal lateral geniculate nucleus (dLGN) receive inputs from retinal ganglion cells via retinogeniculate synapses. These connections undergo pruning in the first 2 weeks after eye opening. The remaining connections are strengthened several-fold by the insertion of AMPA receptors (AMPARs) into weak or silent synapses. In this study, we found that the AMPAR auxiliary subunit CKAMP44 is required for receptor insertion and function of retinogeniculate synapses during development. Genetic deletion of CKAMP44 resulted in decreased synaptic strength and a higher number of silent synapses in young (P9–11) mice. Recovery from desensitisation of AMPARs was faster in CKAMP44 knockout (CKAMP44–/–) than in wild-type mice. Moreover, loss of CKAMP44 increased the probability of inducing plateau potentials, which are known to be important for eye-specific input segregation and retinogeniculate synapse maturation. The anatomy of relay neurons in the dLGN was changed in young CKAMP44–/– mice showing a transient increase in dendritic branching that normalised during later development (P26–33). Interestingly, input segregation in young CKAMP44–/– mice was not affected when compared to wild-type mice. These results demonstrate that CKAMP44 promotes maturation and modulates function of retinogeniculate synapses during early development of the visual system without affecting input segregation. |
DDC-Sachgruppe: | 610 Medizin 610 Medical sciences |
Veröffentlichende Institution: | Johannes Gutenberg-Universität Mainz |
Organisationseinheit: | FB 04 Medizin |
Veröffentlichungsort: | Mainz |
ROR: | https://ror.org/023b0x485 |
DOI: | http://doi.org/10.25358/openscience-8586 |
Version: | Published version |
Publikationstyp: | Zeitschriftenaufsatz |
Nutzungsrechte: | CC BY-NC |
Informationen zu den Nutzungsrechten: | https://creativecommons.org/licenses/by-nc/4.0/ |
Zeitschrift: | The journal of physiology 600 15 |
Seitenzahl oder Artikelnummer: | 3549 3565 |
Verlag: | Wiley-Blackwell |
Verlagsort: | Hoboken, NJ |
Erscheinungsdatum: | 2022 |
ISSN: | 1469-7793 |
DOI der Originalveröffentlichung: | 10.1113/JP283172 |
Enthalten in den Sammlungen: | DFG-491381577-H |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | ||
---|---|---|---|---|---|
ckamp44_controls_synaptic_fun-20230118115938494.pdf | 2.72 MB | Adobe PDF | Öffnen/Anzeigen |