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Authors: Probst, Hans Christian
Stoitzner, Patrizia
Amon, Lukas
Backer, Ronald A.
Brand, Anna
Chen, Jianzhou
Clausen, Björn E.
Dieckmann, Sophie
Dudziak, Diana
Heger, Lukas
Hodapp, Katrin
Hornsteiner, Florian
Hovav, Avi-Hai
Jacobi, Lukas
Ji, Xingqi
Kamenjarin, Nadine
Lahl, Katharina
Lahmar, Imran
Lakus, Jelena
Lehmann, Christian H. K.
Ortner, Daniela
Picard, Marion
Roberti, Maria Paula
Rossnagel, Lukas
Saba, Yasmin
Schalla, Carmen
Schlitzer, Andreas
Schraml, Barbara U.
Schütze, Kristian
Seichter, Anna
Seré, Kristin
Seretis, Athanasios
Sopper, Sieghart
Strandt, Helen
Sykora, Martina M.
Theobald, Hannah
Tripp, Christoph H.
Zitvogel, Laurence
Title: Guidelines for DC preparation and flow cytometry analysis of mouse nonlymphoid tissues
Online publication date: 25-Jan-2023
Year of first publication: 2022
Language: english
Abstract: This article is part of the Dendritic Cell Guidelines article series, which provides a collection of state-of-the-art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy and functional characterization of mouse and human dendritic cells (DC) from lymphoid organs and various nonlymphoid tissues. DC are sentinels of the immune system present in almost every mammalian organ. Since they represent a rare cell population, DC need to be extracted from organs with protocols that are specifically developed for each tissue. This article provides detailed protocols for the preparation of single-cell suspensions from various mouse nonlymphoid tissues, including skin, intestine, lung, kidney, mammary glands, oral mucosa and transplantable tumors. Furthermore, our guidelines include comprehensive protocols for multiplex flow cytometry analysis of DC subsets and feature top tricks for their proper discrimination from other myeloid cells. With this collection, we provide guidelines for in-depth analysis of DC subsets that will advance our understanding of their respective roles in healthy and diseased tissues. While all protocols were written by experienced scientists who routinely use them in their work, this article was also peer-reviewed by leading experts and approved by all coauthors, making it an essential resource for basic and clinical DC immunologists.
DDC: 610 Medizin
610 Medical sciences
Institution: Johannes Gutenberg-Universität Mainz
Department: FB 04 Medizin
Place: Mainz
Version: Published version
Publication type: Zeitschriftenaufsatz
License: CC BY-NC-ND
Information on rights of use:
Journal: European journal of immunology
Version of Record (VoR)
Publisher: Wiley-VCH
Publisher place: Weinheim
Issue date: 2022
ISSN: 1521-4141
Publisher DOI: 10.1002/eji.202249819
Appears in collections:DFG-491381577-H

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