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Authors: Jerominek, Markus
Bull-Hereñu, Kester
Arndt, Melanie
Claßen-Bockhoff, Regine
Title: Live imaging of developmental processes in a living meristem of Davidia involucrata (Nyssaceae)
Online publication date: 21-Oct-2022
Year of first publication: 2014
Language: english
Abstract: Morphogenesis in plants is usually reconstructed by scanning electron microscopy and histology of meristematic structures. These techniques are destructive and require many samples to obtain a consecutive series of states. Unfortunately, using this methodology the absolute timing of growth and complete relative initiation of organs remain obscure. To overcome this limitation, an in vivo observational method based on Epi-Illumination Light Microscopy (ELM) was developed and tested with a male inflorescence meristem (floral unit) of the handkerchief tree Davidia involucrata Baill. (Nyssaceae). We asked whether the most basal flowers of this floral unit arise in a basipetal sequence or, alternatively, are delayed in their development. The growing meristem was observed for 30 days, the longest live observation of a meristem achieved to date. The sequence of primordium initiation indicates a later initiation of the most basal flowers and not earlier or simultaneously as SEM images could suggest. D. involucrata exemplarily shows that live-ELM gives new insights into developmental processes of plants. In addition to morphogenetic questions such as the transition from vegetative to reproductive meristems or the absolute timing of ontogenetic processes, this method may also help to quantify cellular growth processes in the context of molecular physiology and developmental genetics studies.
DDC: 580 Pflanzen (Botanik)
580 Botanical sciences
Institution: Johannes Gutenberg-Universität Mainz
Department: FB 10 Biologie
Place: Mainz
Version: Published version
Publication type: Zeitschriftenaufsatz
License: CC BY
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Journal: Frontiers in plant science
Pages or article number: Art. 613
Publisher: Frontiers Media
Publisher place: Lausanne
Issue date: 2014
ISSN: 1664-462X
Publisher URL:
Publisher DOI: 10.3389/fpls.2014.00613
Appears in collections:DFG-OA-Publizieren (2012 - 2017)

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