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http://doi.org/10.25358/openscience-804
Autoren: | Vancura, Patrick Csicsely, Erika Leiser, Annalisa Iuvone, P. Michael Spessert, Rainer |
Titel: | Rhythmic regulation of photoreceptor and RPE genes important for vision and genetically associated with severe retinal diseases |
Online-Publikationsdatum: | 9-Aug-2018 |
Erscheinungsdatum: | 2018 |
Sprache des Dokuments: | Englisch |
Zusammenfassung/Abstract: | Purpose: The aim of the present study was to identify candidate genes for mediating daily adjustment of vision. Methods: Genes important for vision and genetically associated with severe retinal diseases were tested for 24-hour rhythms in transcript levels in neuronal retina, microdissected photoreceptors, photoreceptor-related pinealocytes, and retinal pigment epithelium-choroid (RPE-choroid) complex by using quantitative PCR. Results: Photoreceptors of wildtype mice display circadian clock-dependent regulation of visual arrestins (Arr1, Arr4) and the visual cycle gene Rdh12, whereas cells of the RPE-choroid exhibit light-dependent regulation of the visual cycle key genes Lrat, Rpe65, and Rdh5. Clock-driven rhythmicity of Arr1, Arr4, and Rdh12 was observed also in rat pinealocytes, to persist in a mouse model of diabetic retinopathy (db/db) and, in the case of Arr1, to be abolished in retinae of mice deficient for dopamine D4 receptors. Therefore, the expression rhythms appear to be evolutionary conserved, to be unaffected in diabetic retinopathy, and, for Arr1, to require dopamine signaling via dopamine D4 receptors. Conclusions: The data of the present study suggest that daily adjustment of retinal function combines clock dependent regulation of genes responsible for phototransduction termination (Arr1, Arr4) and detoxification (Rdh12) in photoreceptors with light-dependent regulation of genes responsible for retinoid recycling (Lrat, Rpe65, and Rdh5) in RPE. Furthermore, they indicate circadian and light dependent regulation of genes genetically associated with severe retinal diseases. |
DDC-Sachgruppe: | 570 Biowissenschaften 570 Life sciences |
Veröffentlichende Institution: | Johannes Gutenberg-Universität Mainz |
Organisationseinheit: | FB 04 Medizin |
Veröffentlichungsort: | Mainz |
ROR: | https://ror.org/023b0x485 |
DOI: | http://doi.org/10.25358/openscience-804 |
URN: | urn:nbn:de:hebis:77-publ-584194 |
Version: | Published version |
Publikationstyp: | Zeitschriftenaufsatz |
Nutzungsrechte: | CC BY-NC-ND |
Informationen zu den Nutzungsrechten: | https://creativecommons.org/licenses/by-nc-nd/4.0/ |
Zeitschrift: | Investigative ophthalmology & visual science 59 10 |
Seitenzahl oder Artikelnummer: | 3789 3799 |
Verlag: | ARVO |
Verlagsort: | Rockville, Md. |
Erscheinungsdatum: | 2018 |
ISSN: | 1552-5783 0146-0404 |
URL der Originalveröffentlichung: | http://dx.doi.org/10.1167/iovs.18-24558 |
DOI der Originalveröffentlichung: | 10.1167/iovs.18-24558 |
Enthalten in den Sammlungen: | JGU-Publikationen |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | ||
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58419.pdf | 1.3 MB | Adobe PDF | Öffnen/Anzeigen |