Autoren:	Lüdeke, Steffen Lohner, Philipp Stühn, Lara G. Betschart, Martin U. Huber, Matthias C. Schreiber, Andreas Schiller, Stefan M.
Titel:	Dynamic structural changes and thermodynamics in phase separation processes of an intrinsically disordered–ordered protein model
Online-Publikationsdatum:	24-Okt-2022
Erscheinungsdatum:	2022
Sprache des Dokuments:	Englisch
Zusammenfassung/Abstract:	Elastin-like proteins (ELPs) are biologically important proteins and models for intrinsically disordered proteins (IDPs) and dynamic structural transitions associated with coacervates and liquid–liquid phase transitions. However, the conformational status below and above coacervation temperature and its role in the phase separation process is still elusive. Employing matrix least-squares global Boltzmann fitting of the circular dichroism spectra of the ELPs (VPGVG)20, (VPGVG)40, and (VPGVG)60, we found that coacervation occurs sharply when a certain number of repeat units has acquired β-turn conformation (in our sequence setting a threshold of approx. 20 repeat units). The character of the differential scattering of the coacervate suspensions indicated that this fraction of β-turn structure is still retained after polypeptide assembly. Such conformational thresholds may also have a role in other protein assembly processes with implications for the design of protein-based smart materials.
DDC-Sachgruppe:	540 Chemie 540 Chemistry and allied sciences
Veröffentlichende Institution:	Johannes Gutenberg-Universität Mainz
Organisationseinheit:	FB 09 Chemie, Pharmazie u. Geowissensch.
Veröffentlichungsort:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-8023
Version:	Published version
Publikationstyp:	Zeitschriftenaufsatz
Nutzungsrechte:	CC BY
Informationen zu den Nutzungsrechten:	https://creativecommons.org/licenses/by/4.0/
Zeitschrift:	Angewandte Chemie 61 3
Seitenzahl oder Artikelnummer:	e202112738
Verlag:	Wiley-VCH
Verlagsort:	Weinheim
Erscheinungsdatum:	2022
ISSN:	1521-3773
DOI der Originalveröffentlichung:	10.1002/anie.202112738
Enthalten in den Sammlungen:	JGU-Publikationen