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Autoren: Allgayer, Julia
Kitsera, Nataliya
Bartelt, Solveig
Epe, Bernd
Khobta, Andriy
Titel: Widespread transcriptional gene inactivation initiated by a repair intermediate of 8-oxoguanine
Online-Publikationsdatum: 14-Okt-2022
Erscheinungsdatum: 2016
Sprache des Dokuments: Englisch
Zusammenfassung/Abstract: DNA damage can significantly modulate expression of the affected genes either by direct structural interference with transcription components or as a collateral outcome of cellular repair attempts. Thus, DNA glycosylases of the base excision repair (BER) pathway have been implicated in negative transcriptional response to several spontaneously generated DNA base modifications, including a common oxidative DNA base modification 8-oxoguanine (8-oxoG). Here, we report that single 8-oxoG situated in the non-transcribed DNA strand of a reporter gene has a pronounced negative effect on transcription, driven by promoters of various strength and with different structural properties, including viral, human, and artificial promoters. We further show that the magnitude of the negative effect on the gene expression correlates with excision of the modified base by OGG1 in all promoter constructs tested. Moreover, by using expression vectors with nuclease resistant backbone modifications, we demonstrate that OGG1 does not catalyse DNA strand cleavage in vivo. Rather, cleavage of the phosphate bond 5′ to 8-oxodG (catalysed by APE1) is essential and universally required for the onset of transcriptional silencing, regardless of the promoter structure. Hence, induction of transcriptional silencing emerges as a ubiquitous mode of biological response to 8-oxoG in DNA.
DDC-Sachgruppe: 610 Medizin
610 Medical sciences
Veröffentlichende Institution: Johannes Gutenberg-Universität Mainz
Organisationseinheit: FB 09 Chemie, Pharmazie u. Geowissensch.
Veröffentlichungsort: Mainz
Version: Published version
Publikationstyp: Zeitschriftenaufsatz
Nutzungsrechte: CC BY-NC
Informationen zu den Nutzungsrechten:
Zeitschrift: Nucleic acids research
Seitenzahl oder Artikelnummer: 7267
Verlag: Oxford Univ. Press
Verlagsort: Oxford
Erscheinungsdatum: 2016
ISSN: 1362-4962
URL der Originalveröffentlichung:
DOI der Originalveröffentlichung: 10.1093/nar/gkw473
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