Authors:	Allgayer, Julia Kitsera, Nataliya Bartelt, Solveig Epe, Bernd Khobta, Andriy
Title:	Widespread transcriptional gene inactivation initiated by a repair intermediate of 8-oxoguanine
Online publication date:	14-Oct-2022
Year of first publication:	2016
Language:	english
Abstract:	DNA damage can significantly modulate expression of the affected genes either by direct structural interference with transcription components or as a collateral outcome of cellular repair attempts. Thus, DNA glycosylases of the base excision repair (BER) pathway have been implicated in negative transcriptional response to several spontaneously generated DNA base modifications, including a common oxidative DNA base modification 8-oxoguanine (8-oxoG). Here, we report that single 8-oxoG situated in the non-transcribed DNA strand of a reporter gene has a pronounced negative effect on transcription, driven by promoters of various strength and with different structural properties, including viral, human, and artificial promoters. We further show that the magnitude of the negative effect on the gene expression correlates with excision of the modified base by OGG1 in all promoter constructs tested. Moreover, by using expression vectors with nuclease resistant backbone modifications, we demonstrate that OGG1 does not catalyse DNA strand cleavage in vivo. Rather, cleavage of the phosphate bond 5′ to 8-oxodG (catalysed by APE1) is essential and universally required for the onset of transcriptional silencing, regardless of the promoter structure. Hence, induction of transcriptional silencing emerges as a ubiquitous mode of biological response to 8-oxoG in DNA.
DDC:	610 Medizin 610 Medical sciences
Institution:	Johannes Gutenberg-Universität Mainz
Department:	FB 09 Chemie, Pharmazie u. Geowissensch.
Place:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-7999
Version:	Published version
Publication type:	Zeitschriftenaufsatz
License:	CC BY-NC
Information on rights of use:	https://creativecommons.org/licenses/by-nc/4.0/
Journal:	Nucleic acids research 44 15
Pages or article number:	7267 7280
Publisher:	Oxford Univ. Press
Publisher place:	Oxford
Issue date:	2016
ISSN:	1362-4962 0305-1048
Publisher URL:	http://dx.doi.org/10.1093/nar/gkw473
Publisher DOI:	10.1093/nar/gkw473
Appears in collections:	DFG-OA-Publizieren (2012 - 2017)