Please use this identifier to cite or link to this item:
http://doi.org/10.25358/openscience-7996
Authors: | Shen, Limei Higuchi, Tetsuya Tubbe, Ingrid Voltz, Nicole Krummen, Mathias Pektor, Stefanie Montermann, Evelyn Rausch, Kristin Schmidt, Manfred Schild, Hansjörg Grabbe, Stephan Bros, Matthias |
Title: | A trifunctional dextran-based nanovaccine targets and activates murine dendritic cells, and induces potent cellular and humoral immune responses in vivo |
Online publication date: | 14-Oct-2022 |
Year of first publication: | 2013 |
Language: | english |
Abstract: | Dendritic cells (DCs) constitute an attractive target for specific delivery of nanovaccines for immunotherapeutic applications. Here we tested nano-sized dextran (DEX) particles to serve as a DC-addressing nanocarrier platform. Non-functionalized DEX particles had no immunomodulatory effect on bone marrow (BM)-derived murine DCs in vitro. However, when adsorbed with ovalbumine (OVA), DEX particles were efficiently engulfed by BM-DCs in a mannose receptor-dependent manner. A DEX-based nanovaccine containing OVA and lipopolysaccharide (LPS) as a DC stimulus induced strong OVA peptide-specific CD4(+) and CD8(+) T cell proliferation both in vitro and upon systemic application in mice, as well as a robust OVA-specific humoral immune response (IgG1>IgG2a) in vivo. Accordingly, this nanovaccine also raised both a more pronounced delayed-type hypersensitivity response and a stronger induction of cytotoxic CD8(+) T cells than obtained upon administration of OVA and LPS in soluble form. Therefore, DEX-based nanoparticles constitute a potent, versatile and easy to prepare nanovaccine platform for immunotherapeutic approaches. |
DDC: | 610 Medizin 610 Medical sciences |
Institution: | Johannes Gutenberg-Universität Mainz |
Department: | FB 04 Medizin FB 09 Chemie, Pharmazie u. Geowissensch. |
Place: | Mainz |
ROR: | https://ror.org/023b0x485 |
DOI: | http://doi.org/10.25358/openscience-7996 |
Version: | Published version |
Publication type: | Zeitschriftenaufsatz |
License: | CC BY |
Information on rights of use: | https://creativecommons.org/licenses/by/3.0/ |
Journal: | PLoS one 8 12 |
Pages or article number: | e80904 |
Publisher: | PLoS |
Publisher place: | Lawrence, Kan. |
Issue date: | 2013 |
ISSN: | 1932-6203 |
Publisher URL: | http://dx.doi.org/10.1371/journal.pone.0080904 |
Publisher DOI: | 10.1371/journal.pone.0080904 |
Appears in collections: | DFG-OA-Publizieren (2012 - 2017) |
Files in This Item:
File | Description | Size | Format | ||
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a_trifunctional_dextranbased_-20220925162029392.pdf | 1.73 MB | Adobe PDF | View/Open |