Authors:	Gonsior, Constantin Binamé, Fabien Frühbeis, Carsten Bauer, Nina M. Hoch-Kraft, Peter Luhmann, Heiko Trotter, Jacqueline White, Robin
Title:	Oligodendroglial p130Cas is a target of Fyn kinase involved in process formation, cell migration and survival
Online publication date:	6-Oct-2022
Year of first publication:	2014
Language:	english
Abstract:	Oligodendrocytes are the myelinating glial cells of the central nervous system. In the course of brain development, oligodendrocyte precursor cells migrate, scan the environment and differentiate into mature oligodendrocytes with multiple cellular processes which recognize and ensheath neuronal axons. During differentiation, oligodendrocytes undergo dramatic morphological changes requiring cytoskeletal rearrangements which need to be tightly regulated. The non-receptor tyrosine kinase Fyn plays a central role in oligodendrocyte differentiation and myelination. In order to improve our understanding of the role of oligodendroglial Fyn kinase, we have identified Fyn targets in these cells. Purification and mass-spectrometric analysis of tyrosine-phosphorylated proteins in response to overexpressed active Fyn in the oligodendrocyte precursor cell line Oli-neu, yielded the adaptor molecule p130Cas. We analyzed the function of this Fyn target in oligodendroglial cells and observed that reduction of p130Cas levels by siRNA affects process outgrowth, the thickness of cellular processes and migration behavior of Oli-neu cells. Furthermore, long term p130Cas reduction results in decreased cell numbers as a result of increased apoptosis in cultured primary oligodendrocytes. Our data contribute to understanding the molecular events taking place during oligodendrocyte migration and morphological differentiation and have implications for myelin formation.
DDC:	610 Medizin 610 Medical sciences
Institution:	Johannes Gutenberg-Universität Mainz
Department:	FB 04 Medizin FB 10 Biologie
Place:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-7883
Version:	Published version
Publication type:	Zeitschriftenaufsatz
License:	CC BY
Information on rights of use:	https://creativecommons.org/licenses/by/4.0/
Journal:	PLoS one 9 2
Pages or article number:	e89423
Publisher:	PLoS
Publisher place:	Lawrence, Kan.
Issue date:	2014
ISSN:	1932-6203
Publisher URL:	http://dx.doi.org/10.1371/journal.pone.0089423
Publisher DOI:	10.1371/journal.pone.0089423
Appears in collections:	DFG-OA-Publizieren (2012 - 2017)