Authors:	Ding, Rouven Weynans, Kevin Bossing, Torsten Barros, Claudia S. Berger, Christian
Title:	The Hippo signalling pathway maintains quiescence in Drosophila neural stem cells
Online publication date:	6-Oct-2022
Year of first publication:	2016
Language:	english
Abstract:	Stem cells control their mitotic activity to decide whether to proliferate or to stay in quiescence. Drosophila neural stem cells (NSCs) are quiescent at early larval stages, when they are reactivated in response to metabolic changes. Here we report that cell-contact inhibition of growth through the canonical Hippo signalling pathway maintains NSC quiescence. Loss of the core kinases hippo or warts leads to premature nuclear localization of the transcriptional co-activator Yorkie and initiation of growth and proliferation in NSCs. Yorkie is necessary and sufficient for NSC reactivation, growth and proliferation. The Hippo pathway activity is modulated via inter-cellular transmembrane proteins Crumbs and Echinoid that are both expressed in a nutrient-dependent way in niche glial cells and NSCs. Loss of crumbs or echinoid in the niche only is sufficient to reactivate NSCs. Finally, we provide evidence that the Hippo pathway activity discriminates quiescent from non-quiescent NSCs in the Drosophila nervous system.
DDC:	570 Biowissenschaften 570 Life sciences
Institution:	Johannes Gutenberg-Universität Mainz
Department:	FB 10 Biologie
Place:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-7876
Version:	Published version
Publication type:	Zeitschriftenaufsatz
License:	CC BY
Information on rights of use:	https://creativecommons.org/licenses/by/4.0/
Journal:	Nature communications 7
Pages or article number:	Art. 10510
Publisher:	Nature Publishing Group
Publisher place:	London
Issue date:	2016
ISSN:	2041-1723
Publisher URL:	http://dx.doi.org/10.1038/ncomms10510
Publisher DOI:	10.1038/ncomms10510
Appears in collections:	DFG-OA-Publizieren (2012 - 2017)