Please use this identifier to cite or link to this item:
http://doi.org/10.25358/openscience-7868
Authors: | Halbroth, Benedict R. Heil, Alexander Distler, Eva Dass, Martin Wagner, Eva Maria Plachter, Bodo Probst, Hans Christian Strand, Dennis Hartwig, Udo Karner, Anita Aichinger, Gerald Kistner, Otfried Landfester, Katharina Herr, Wolfgang |
Title: | Superior in vitro stimulation of human CD8+ T-cells by whole virus versus split virus influenza vaccines |
Online publication date: | 6-Oct-2022 |
Year of first publication: | 2014 |
Language: | english |
Abstract: | Pandemic and seasonal influenza viruses cause considerable morbidity and mortality in the general human population. Protection from severe disease may result from vaccines that activate antigen-presenting DC for effective stimulation of influenza-specific memory T cells. Special attention is paid to vaccine-induced CD8+ T-cell responses, because they are mainly directed against conserved internal influenza proteins thereby presumably mediating cross-protection against circulating seasonal as well as emerging pandemic virus strains. Our study showed that influenza whole virus vaccines of major seasonal A and B strains activated DC more efficiently than those of pandemic swine-origin H1N1 and pandemic-like avian H5N1 strains. In contrast, influenza split virus vaccines had a low ability to activate DC, regardless which strain was investigated. We also observed that whole virus vaccines stimulated virus-specific CD8+ memory T cells much stronger compared to split virus counterparts, whereas both vaccine formats activated CD4+ Th cell responses similarly. Moreover, our data showed that whole virus vaccine material is delivered into the cytosolic pathway of DC for effective activation of virus-specific CD8+ T cells. We conclude that vaccines against seasonal and pandemic (-like) influenza strains that aim to stimulate cross-reacting CD8+ T cells should include whole virus rather than split virus formulations. |
DDC: | 610 Medizin 610 Medical sciences |
Institution: | Johannes Gutenberg-Universität Mainz |
Department: | FB 04 Medizin |
Place: | Mainz |
ROR: | https://ror.org/023b0x485 |
DOI: | http://doi.org/10.25358/openscience-7868 |
Version: | Published version |
Publication type: | Zeitschriftenaufsatz |
License: | CC BY |
Information on rights of use: | https://creativecommons.org/licenses/by/4.0/ |
Journal: | PLoS one 9 7 |
Pages or article number: | e103392 |
Publisher: | PLoS |
Publisher place: | Lawrence, Kan. |
Issue date: | 2014 |
ISSN: | 1932-6203 |
Publisher URL: | http://dx.doi.org/10.1371/journal.pone.0103392 |
Publisher DOI: | 10.1371/journal.pone.0103392 |
Appears in collections: | DFG-OA-Publizieren (2012 - 2017) |
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