Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-7853
Authors: Wilding, Corina
Bell, Katharina
Funke, S.
Beck, Sabine
Pfeiffer, Norbert
Grus, Franz-Hermann
Title: GFAP antibodies show protective effect on oxidatively stressed neuroretinal cells via interaction with ERP57
Online publication date: 5-Oct-2022
Year of first publication: 2015
Language: english
Abstract: The pathogenesis of glaucoma, a common neurodegenerative disease, involves an immunologic component. Changes in the natural autoantibody profile of glaucoma patients were detected, showing not only up-regulated but also down-regulated immunoreactivities. In recent studies we were able to demonstrate that the antibody changes have a large influence on protein profiles of neuroretinal cells. Furthermore we could demonstrate neuroprotective potential of one of the down-regulated antibodies (gamma-synuclein antibody). Anti-GFAP antibody is another antibody found down-regulated in glaucoma patients. Since GFAP expression is intensified in glaucomatous retina, the aim of this study was to detect the effect of GFAP antibodies on neuroretinal cells. This is realized with a viability-test as well as proteomic analysis of cells incubated with GFAP antibodies. Furthermore, possible interaction partners of the GFAP antibody in neuroretinal cells were identified by western blot, mass spectrometry and indirect immunofluorescence staining. We found that the GFAP antibody is able to protect cells from oxidative stress, which is due to changed protein expressions of the actin cytoskeleton. Furthermore we detected a cross-reaction of the antibody to endoplasmic reticulum resident protein 57 on the cell membrane, which seems to lead to a changed signaling in the cells triggering the protective effects.
DDC: 610 Medizin
610 Medical sciences
Institution: Johannes Gutenberg-Universität Mainz
Department: FB 04 Medizin
Place: Mainz
ROR: https://ror.org/023b0x485
DOI: http://doi.org/10.25358/openscience-7853
Version: Published version
Publication type: Zeitschriftenaufsatz
License: CC BY-NC-ND
Information on rights of use: https://creativecommons.org/licenses/by-nc-nd/4.0/
Journal: Journal of pharmacological sciences
127
3
Pages or article number: 298
304
Publisher: The Japanese Pharmacological Society
Publisher place: Tokyo
Issue date: 2015
ISSN: 1347-8648
1347-8613
Publisher URL: http://dx.doi.org/10.1016/j.jphs.2014.12.019
Publisher DOI: 10.1016/j.jphs.2014.12.019
Appears in collections:DFG-OA-Publizieren (2012 - 2017)

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