Please use this identifier to cite or link to this item:
http://doi.org/10.25358/openscience-7853
Authors: | Wilding, Corina Bell, Katharina Funke, S. Beck, Sabine Pfeiffer, Norbert Grus, Franz-Hermann |
Title: | GFAP antibodies show protective effect on oxidatively stressed neuroretinal cells via interaction with ERP57 |
Online publication date: | 5-Oct-2022 |
Year of first publication: | 2015 |
Language: | english |
Abstract: | The pathogenesis of glaucoma, a common neurodegenerative disease, involves an immunologic component. Changes in the natural autoantibody profile of glaucoma patients were detected, showing not only up-regulated but also down-regulated immunoreactivities. In recent studies we were able to demonstrate that the antibody changes have a large influence on protein profiles of neuroretinal cells. Furthermore we could demonstrate neuroprotective potential of one of the down-regulated antibodies (gamma-synuclein antibody). Anti-GFAP antibody is another antibody found down-regulated in glaucoma patients. Since GFAP expression is intensified in glaucomatous retina, the aim of this study was to detect the effect of GFAP antibodies on neuroretinal cells. This is realized with a viability-test as well as proteomic analysis of cells incubated with GFAP antibodies. Furthermore, possible interaction partners of the GFAP antibody in neuroretinal cells were identified by western blot, mass spectrometry and indirect immunofluorescence staining. We found that the GFAP antibody is able to protect cells from oxidative stress, which is due to changed protein expressions of the actin cytoskeleton. Furthermore we detected a cross-reaction of the antibody to endoplasmic reticulum resident protein 57 on the cell membrane, which seems to lead to a changed signaling in the cells triggering the protective effects. |
DDC: | 610 Medizin 610 Medical sciences |
Institution: | Johannes Gutenberg-Universität Mainz |
Department: | FB 04 Medizin |
Place: | Mainz |
ROR: | https://ror.org/023b0x485 |
DOI: | http://doi.org/10.25358/openscience-7853 |
Version: | Published version |
Publication type: | Zeitschriftenaufsatz |
License: | CC BY-NC-ND |
Information on rights of use: | https://creativecommons.org/licenses/by-nc-nd/4.0/ |
Journal: | Journal of pharmacological sciences 127 3 |
Pages or article number: | 298 304 |
Publisher: | The Japanese Pharmacological Society |
Publisher place: | Tokyo |
Issue date: | 2015 |
ISSN: | 1347-8648 1347-8613 |
Publisher URL: | http://dx.doi.org/10.1016/j.jphs.2014.12.019 |
Publisher DOI: | 10.1016/j.jphs.2014.12.019 |
Appears in collections: | DFG-OA-Publizieren (2012 - 2017) |
Files in This Item:
File | Description | Size | Format | ||
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gfap_antibodies_show_protecti-20220914233941934.pdf | 1.19 MB | Adobe PDF | View/Open |