Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-7824
Authors: Baranowski, Andreas
Klein, Anja
Ritz, Ulrike
Ackermann, Angelika
Anthonissen, Joris
Kaufmann, Kerstin B.
Brendel, Christian
Götz, Hermann
Rommens, Pol Maria
Hofmann, Alexander
Title: Surface functionalization of orthopedic titanium implants with bone sialoprotein
Online publication date: 4-Oct-2022
Year of first publication: 2016
Language: english
Abstract: Orthopedic implant failure due to aseptic loosening and mechanical instability remains a major problem in total joint replacement. Improving osseointegration at the bone-implant interface may reduce micromotion and loosening. Bone sialoprotein (BSP) has been shown to enhance bone formation when coated onto titanium femoral implants and in rat calvarial defect models. However, the most appropriate method of BSP coating, the necessary level of BSP coating, and the effect of BSP coating on cell behavior remain largely unknown. In this study, BSP was covalently coupled to titanium surfaces via an aminosilane linker (APTES), and its properties were compared to BSP applied to titanium via physisorption and untreated titanium. Cell functions were examined using primary human osteoblasts (hOBs) and L929 mouse fibroblasts. Gene expression of specific bone turnover markers at the RNA level was detected at different intervals. Cell adhesion to titanium surfaces treated with BSP via physisorption was not significantly different from that of untreated titanium at any time point, whereas BSP application via covalent coupling caused reduced cell adhesion during the first few hours in culture. Cell migration was increased on titanium disks that were treated with higher concentrations of BSP solution, independent of the coating method. During the early phases of hOB proliferation, a suppressive effect of BSP was observed independent of its concentration, particularly when BSP was applied to the titanium surface via physisorption. Although alkaline phosphatase activity was reduced in the BSP-coated titanium groups after 4 days in culture, increased calcium deposition was observed after 21 days. In particular, the gene expression level of RUNX2 was upregulated by BSP. The increase in calcium deposition and the stimulation of cell differentiation induced by BSP highlight its potential as a surface modifier that could enhance the osseointegration of orthopedic implants. Both physisorption and covalent coupling of BSP are similarly effective, feasible methods, although a higher BSP concentration is recommended.
DDC: 610 Medizin
610 Medical sciences
Institution: Johannes Gutenberg-Universität Mainz
Department: FB 04 Medizin
Place: Mainz
ROR: https://ror.org/023b0x485
DOI: http://doi.org/10.25358/openscience-7824
Version: Published version
Publication type: Zeitschriftenaufsatz
License: CC BY
Information on rights of use: https://creativecommons.org/licenses/by/4.0/
Journal: PLoS one
11
4
Pages or article number: e0153978
Publisher: PLoS
Publisher place: Lawrence, Kan.
Issue date: 2016
ISSN: 1932-6203
Publisher URL: http://dx.doi.org/10.1371/journal.pone.0153978
Publisher DOI: 10.1371/journal.pone.0153978
Appears in collections:DFG-OA-Publizieren (2012 - 2017)

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