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Authors: Shen, Limei
Tenzer, Stefan
Hess, Moritz
Distler, Ute
Tubbe, Ingrid
Montermann, Evelyn
Schimmer, Simone
Dittmer, Ulf
Grabbe, Stephan
Bros, Matthias
Title: Friend virus limits adaptive cellular immune responses by imprinting a maturation-resistant and T helper type 2-biased immunophenotype in dendritic cells
Online publication date: 26-Nov-2018
Language: english
Abstract: The murine Friend virus (FV) retrovirus model has been widely used to study anti-viral immune responses, and virus-induced cancer. Here we analyzed FV immune evasion mechanisms on the level of dendritic cells (DC) essential for the induction of primary adaptive immune responses. Comparative quantitative proteome analysis of FV-infected DC (FV-DC) of different genotypes (BALB/c, C57BL/6) and non-infected DC revealed numerous genotype-independently regulated proteins rergulating metabolic activity, cytoskeletal rearrangements, and antigen processing/presentation. These alterations may promote virion production in FV-DC. Stimulation of FV-DC with LPS resulted in strongly enhanced IL-10 production which was partially responsible for their attenuated T cell (CD4 , CD8 ) stimulatory capacity. Stimulated FV-DC induced less IFN-γ production in T cells required for cellular anti-viral responses, but more T helper cell type 2 (Th2)-associated cytokines (IL-4, IL-5, IL-13). We conclude that FV reprograms DC to promote viral spreading and immune deviation by imprinting a largely maturation-resistant, Th2-biased immunophenotype.
DDC: 610 Medizin
610 Medical sciences
Institution: Johannes Gutenberg-Universität Mainz
Department: FB 04 Medizin
Place: Mainz
URN: urn:nbn:de:hebis:77-publ-586564
Version: Published version
Publication type: Zeitschriftenaufsatz
License: CC BY
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Journal: PLOS ONE
Pages or article number: e0192541
Publisher: PLOS
Publisher place: San Francisco, California, US
Issue date: 2018
ISSN: 1932-6203
Publisher URL:
Publisher DOI: 10.1371/journal.pone.0192541
Appears in collections:JGU-Publikationen

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