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Authors: Teister, Julia
Anders, F.
Beck, Sabine
Funke, S.
Pein, H. von
Prokosch-Willing, Verena
Pfeiffer, Norbert
Grus, Franz-Hermann
Title: Decelerated neurodegeneration after intravitreal injection of α-synuclein antibodies in a glaucoma animal model
Online publication date: 19-Aug-2022
Year of first publication: 2017
Language: english
Abstract: Although elevated intraocular pressure (IOP) remains the major risk factor in glaucoma, neurodegenerative processes continue despite effective IOP lowering. Altered α-synuclein antibody (Abs) levels have been reported to play a crucial role. This study aimed at identifying whether α-synuclein Abs are capable to decelerate neuronal decay while providing insights into proteomic changes. Four groups of Sprague Dawley rats received episcleral vein occlusion: (1) CTRL, no intravitreal injection, n = 6, (2) CTRL IgG, intravitreal injection of unspecific IgG, n = 5, (3) Buffer, intravitreal injection of buffer, n = 6, (4), α-synuclein Ab, intravitreal injection of α-synuclein Ab, n = 5. IOP and retinal nerve fiber layer thickness (RNFLT) were monitored and immunohistochemistry, microarray and proteomic analysis were performed. RNFLT was reduced in CTRL, CTRL IgG and Buffer group (all p < 0.01) and α-synuclein Ab group (p = 0.17). Axon and RGC density showed an increased neurodegeneration in CTRL, CTRL IgG and Buffer group (all p < 0.01) and increased neuronal survival in α-synuclein Ab group (p = 0.38 and 0.06, respectively) compared with fellow eyes. Proteomic analysis revealed alterations of cofilin 1 and superoxide dismutase 1 expression. This data indicate that α-synuclein Ab might indirectly modulate the actin cytoskeleton organization and negatively regulate apoptotic processes via cofilin 1 and superoxide dismutase 1.
DDC: 610 Medizin
610 Medical sciences
Institution: Johannes Gutenberg-Universität Mainz
Department: FB 04 Medizin
Place: Mainz
Version: Published version
Publication type: Zeitschriftenaufsatz
License: CC BY
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Journal: Scientific reports
Pages or article number: Art. 6260
Publisher: Macmillan Publishers Limited, part of Springer Nature
Publisher place: London
Issue date: 2017
ISSN: 2045-2322
Publisher URL:
Publisher DOI: 10.1038/s41598-017-06702-1
Annotation: Prokosch-Willing, Verena veröffentlicht unter: Prokosch, Verena
Appears in collections:DFG-OA-Publizieren (2012 - 2017)

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