Gutenberg Open Science: De novo transcriptome assembly and comparison of C3, C3-C4, and C4 species of tribe Salsoleae (Chenopodiaceae)

Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-7364

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DC Field	Value	Language
dc.contributor.author	Lauterbach, Maximilian	-
dc.contributor.author	Schmidt, Hanno	-
dc.contributor.author	Billakurthi, Kumari	-
dc.contributor.author	Hankeln, Thomas	-
dc.contributor.author	Westhoff, Peter	-
dc.contributor.author	Gowik, Udo	-
dc.contributor.author	Kadereit, Gudrun	-
dc.date.accessioned	2022-07-12T07:30:47Z	-
dc.date.available	2022-07-12T07:30:47Z	-
dc.date.issued	2017
dc.identifier.uri	https://openscience.ub.uni-mainz.de/handle/20.500.12030/7378	-
dc.description.abstract	C4 photosynthesis is a carbon-concentrating mechanism that evolved independently more than 60 times in a wide range of angiosperm lineages. Among other alterations, the evolution of C4 from ancestral C3 photosynthesis requires changes in the expression of a vast number of genes. Differential gene expression analyses between closely related C3 and C4 species have significantly increased our understanding of C4 functioning and evolution. In Chenopodiaceae, a family that is rich in C4 origins and photosynthetic types, the anatomy, physiology and phylogeny of C4, C2 and C3 species of Salsoleae has been studied in great detail, which facilitated the choice of six samples of five representative species with different photosynthetic types for transcriptome comparisons. mRNA from assimilating organs of each species was sequenced in triplicates, and sequence reads were de novo assembled. These novel genetic resources were then analyzed to provide a better understanding of differential gene expression between C3, C2 and C4 species. All three analyzed C4 species belong to the NADP-ME type as most genes encoding core enzymes of this C4 cycle are highly expressed. The abundance of photorespiratory transcripts is decreased compared to the C3 and C2 species. Like in other C4 lineages of Caryophyllales, our results suggest that PEPC1 is the C4-specific isoform in Salsoleae. Two recently identified transporters from the PHT4 protein family may not only be related to the C4 syndrome, but also active in C2 photosynthesis in Salsoleae. In the two populations of the C2 species S. divaricata transcript abundance of several C4 genes are slightly increased, however a C4 cycle is not detectable in the carbon isotope values. Most of the core enzymes of photorespiration are highly increased in the C2 species compared to both C3 and C4 species, confirming a successful establishment of the C2 photosynthetic pathway. Furthermore, a function of PEP-CK in C2 photosynthesis appears likely, since PEP-CK gene expression is not only increased in S. divaricata but also in C2 species of other groups.	en_GB
dc.description.sponsorship	DFG, Open Access-Publizieren Universität Mainz / Universitätsmedizin	de
dc.language.iso	eng	de
dc.rights	CC BY	*
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/	*
dc.subject.ddc	570 Biowissenschaften	de_DE
dc.subject.ddc	570 Life sciences	en_GB
dc.title	De novo transcriptome assembly and comparison of C3, C3-C4, and C4 species of tribe Salsoleae (Chenopodiaceae)	en_GB
dc.type	Zeitschriftenaufsatz	de
dc.identifier.doi	http://doi.org/10.25358/openscience-7364	-
jgu.type.dinitype	article	en_GB
jgu.type.version	Published version	de
jgu.type.resource	Text	de
jgu.organisation.department	FB 10 Biologie	de
jgu.organisation.number	7970	-
jgu.organisation.name	Johannes Gutenberg-Universität Mainz	-
jgu.rights.accessrights	openAccess	-
jgu.journal.title	Frontiers in plant science	de
jgu.journal.volume	8	de
jgu.pages.alternative	Art. 1939	de
jgu.publisher.year	2017	-
jgu.publisher.name	Frontiers Media	de
jgu.publisher.place	Lausanne	de
jgu.publisher.uri	http://dx.doi.org/10.3389/fpls.2017.01939	de
jgu.publisher.issn	1664-462X	de
jgu.organisation.place	Mainz	-
jgu.subject.ddccode	570	de
opus.date.modified	2018-04-26T10:56:26Z
opus.subject.dfgcode	00-000
opus.organisation.string	FB 10: Biologie: Institut für Molekulare Physiologie	de_DE
opus.organisation.string	FB 10: Biologie: Institut für Organismische und Molekulare Evolutionsbiologie	de_DE
opus.identifier.opusid	58116
opus.institute.number	1013
opus.institute.number	1011
opus.metadataonly	false
opus.type.contenttype	Keine	de_DE
opus.type.contenttype	None	en_EN
opus.affiliated	Lauterbach, Maximilian
opus.affiliated	Hankeln, Thomas
opus.affiliated	Kadereit, Gudrun
jgu.publisher.doi	10.3389/fpls.2017.01939	de
jgu.organisation.ror	https://ror.org/023b0x485
Appears in collections:	DFG-OA-Publizieren (2012 - 2017)

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