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Autoren: Lauterbach, Maximilian
Schmidt, Hanno
Billakurthi, Kumari
Hankeln, Thomas
Westhoff, Peter
Gowik, Udo
Kadereit, Gudrun
Titel: De novo transcriptome assembly and comparison of C3, C3-C4, and C4 species of tribe Salsoleae (Chenopodiaceae)
Online-Publikationsdatum: 12-Jul-2022
Erscheinungsdatum: 2017
Sprache des Dokuments: Englisch
Zusammenfassung/Abstract: C4 photosynthesis is a carbon-concentrating mechanism that evolved independently more than 60 times in a wide range of angiosperm lineages. Among other alterations, the evolution of C4 from ancestral C3 photosynthesis requires changes in the expression of a vast number of genes. Differential gene expression analyses between closely related C3 and C4 species have significantly increased our understanding of C4 functioning and evolution. In Chenopodiaceae, a family that is rich in C4 origins and photosynthetic types, the anatomy, physiology and phylogeny of C4, C2 and C3 species of Salsoleae has been studied in great detail, which facilitated the choice of six samples of five representative species with different photosynthetic types for transcriptome comparisons. mRNA from assimilating organs of each species was sequenced in triplicates, and sequence reads were de novo assembled. These novel genetic resources were then analyzed to provide a better understanding of differential gene expression between C3, C2 and C4 species. All three analyzed C4 species belong to the NADP-ME type as most genes encoding core enzymes of this C4 cycle are highly expressed. The abundance of photorespiratory transcripts is decreased compared to the C3 and C2 species. Like in other C4 lineages of Caryophyllales, our results suggest that PEPC1 is the C4-specific isoform in Salsoleae. Two recently identified transporters from the PHT4 protein family may not only be related to the C4 syndrome, but also active in C2 photosynthesis in Salsoleae. In the two populations of the C2 species S. divaricata transcript abundance of several C4 genes are slightly increased, however a C4 cycle is not detectable in the carbon isotope values. Most of the core enzymes of photorespiration are highly increased in the C2 species compared to both C3 and C4 species, confirming a successful establishment of the C2 photosynthetic pathway. Furthermore, a function of PEP-CK in C2 photosynthesis appears likely, since PEP-CK gene expression is not only increased in S. divaricata but also in C2 species of other groups.
DDC-Sachgruppe: 570 Biowissenschaften
570 Life sciences
Veröffentlichende Institution: Johannes Gutenberg-Universität Mainz
Organisationseinheit: FB 10 Biologie
Veröffentlichungsort: Mainz
ROR: https://ror.org/023b0x485
DOI: http://doi.org/10.25358/openscience-7364
Version: Published version
Publikationstyp: Zeitschriftenaufsatz
Nutzungsrechte: CC BY
Informationen zu den Nutzungsrechten: https://creativecommons.org/licenses/by/4.0/
Zeitschrift: Frontiers in plant science
8
Seitenzahl oder Artikelnummer: Art. 1939
Verlag: Frontiers Media
Verlagsort: Lausanne
Erscheinungsdatum: 2017
ISSN: 1664-462X
URL der Originalveröffentlichung: http://dx.doi.org/10.3389/fpls.2017.01939
DOI der Originalveröffentlichung: 10.3389/fpls.2017.01939
Enthalten in den Sammlungen:DFG-OA-Publizieren (2012 - 2017)

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