Authors:	Tamaki, Ryo Orie, Samuel Ige Alessandri, Beat Kempski, Oliver Heimann, Axel
Title:	Spreading depression and focal venous cerebral ischemia enhance cortical neurogenesis
Online publication date:	13-Jun-2022
Year of first publication:	2017
Language:	english
Abstract:	Endogenous neurogenesis can arise from a variety of physiological stimuli including exercise, learning, or “enriched environment” as well as pathological conditions such as ischemia, epilepsy or cortical spreading depression. Whether all these conditions use a common trigger to set off endogenous neurogenesis is yet unclear. We hypothesized that cortical spreading depression (CSD) induces neurogenesis in the cerebral cortex and dentate gyrus after cerebral venous ischemia. Forty-two Wistar rats alternatively underwent sham operation (Sham), induction of ten CSDs or venous ischemia provoked via occlusion of two adjacent superficial cortical vein followed by ten induced CSDs (CSD + 2-VO). As an additional control, 15 naïve rats received no intervention except 5-bromo-2′-deoxyuridine (BrdU) treatment for 7 days. Sagittal brain slices (40 μm thick) were co-stained for BrdU and doublecortin (DCX; new immature neuronal cells) on day 9 or NeuN (new mature neuronal cells) on day 28. On day 9 after sham operation, cell proliferation and neurogenesis occurred in the cortex in rats. The sole induction of CSD had no effect. But on days 9 and 28, more proliferating cells and newly formed neurons in the ipsilateral cortex were observed in rats subjected to CSD + 2VO than in rats subjected to sham operation. On days 9 and 28, cell proliferation and neurogenesis in the ipsilateral dentate gyrus was increased in sham-operated rats than in naïve rats. Our data supports the hypothesis that induced cortical neurogenesis after CSD + 2-VO is a direct effect of ischemia, rather than of CSD alone.
DDC:	610 Medizin 610 Medical sciences
Institution:	Johannes Gutenberg-Universität Mainz
Department:	FB 04 Medizin
Place:	Mainz
ROR:	https://ror.org/023b0x485
DOI:	http://doi.org/10.25358/openscience-7132
Version:	Published version
Publication type:	Zeitschriftenaufsatz
License:	CC BY-NC-SA
Information on rights of use:	https://creativecommons.org/licenses/by-nc-sa/3.0/
Journal:	Neural regeneration research 12 8
Pages or article number:	1278 1286
Publisher:	Wolters Kluwer Health Medknow
Publisher place:	Mumbai
Issue date:	2017
ISSN:	1673-5374 1876-7958
Publisher URL:	http://dx.doi.org/10.4103/1673-5374.213547
Publisher DOI:	10.4103/1673-5374.213547
Appears in collections:	DFG-OA-Publizieren (2012 - 2017)