Please use this identifier to cite or link to this item:
http://doi.org/10.25358/openscience-7132
Authors: | Tamaki, Ryo Orie, Samuel Ige Alessandri, Beat Kempski, Oliver Heimann, Axel |
Title: | Spreading depression and focal venous cerebral ischemia enhance cortical neurogenesis |
Online publication date: | 13-Jun-2022 |
Year of first publication: | 2017 |
Language: | english |
Abstract: | Endogenous neurogenesis can arise from a variety of physiological stimuli including exercise, learning, or “enriched environment” as well as pathological conditions such as ischemia, epilepsy or cortical spreading depression. Whether all these conditions use a common trigger to set off endogenous neurogenesis is yet unclear. We hypothesized that cortical spreading depression (CSD) induces neurogenesis in the cerebral cortex and dentate gyrus after cerebral venous ischemia. Forty-two Wistar rats alternatively underwent sham operation (Sham), induction of ten CSDs or venous ischemia provoked via occlusion of two adjacent superficial cortical vein followed by ten induced CSDs (CSD + 2-VO). As an additional control, 15 naïve rats received no intervention except 5-bromo-2′-deoxyuridine (BrdU) treatment for 7 days. Sagittal brain slices (40 μm thick) were co-stained for BrdU and doublecortin (DCX; new immature neuronal cells) on day 9 or NeuN (new mature neuronal cells) on day 28. On day 9 after sham operation, cell proliferation and neurogenesis occurred in the cortex in rats. The sole induction of CSD had no effect. But on days 9 and 28, more proliferating cells and newly formed neurons in the ipsilateral cortex were observed in rats subjected to CSD + 2VO than in rats subjected to sham operation. On days 9 and 28, cell proliferation and neurogenesis in the ipsilateral dentate gyrus was increased in sham-operated rats than in naïve rats. Our data supports the hypothesis that induced cortical neurogenesis after CSD + 2-VO is a direct effect of ischemia, rather than of CSD alone. |
DDC: | 610 Medizin 610 Medical sciences |
Institution: | Johannes Gutenberg-Universität Mainz |
Department: | FB 04 Medizin |
Place: | Mainz |
ROR: | https://ror.org/023b0x485 |
DOI: | http://doi.org/10.25358/openscience-7132 |
Version: | Published version |
Publication type: | Zeitschriftenaufsatz |
License: | CC BY-NC-SA |
Information on rights of use: | https://creativecommons.org/licenses/by-nc-sa/3.0/ |
Journal: | Neural regeneration research 12 8 |
Pages or article number: | 1278 1286 |
Publisher: | Wolters Kluwer Health Medknow |
Publisher place: | Mumbai |
Issue date: | 2017 |
ISSN: | 1673-5374 1876-7958 |
Publisher URL: | http://dx.doi.org/10.4103/1673-5374.213547 |
Publisher DOI: | 10.4103/1673-5374.213547 |
Appears in collections: | DFG-OA-Publizieren (2012 - 2017) |
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![]() | spreading_depression_and_foca-20220612154823881.pdf | 732.18 kB | Adobe PDF | View/Open |