Gutenberg Open Science: Improved SiRNA loading of cationic nanohydrogel particles by variation of crosslinking density

Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-7011

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DC Field	Value	Language
dc.contributor.author	Leber, Nadine	-
dc.contributor.author	Zentel, Rudolf	-
dc.date.accessioned	2022-05-20T10:19:52Z	-
dc.date.available	2022-05-20T10:19:52Z	-
dc.date.issued	2020	-
dc.identifier.uri	https://openscience.ub.uni-mainz.de/handle/20.500.12030/7025	-
dc.description.abstract	This paper deals with a concept to improve the loading capability of cationic nanohydrogel particles with siRNA. For this purpose, a new tetrafunctional crosslinker is synthesized via a peptide coupling approach using lysine and spermine derivatives. Applying this four-arm crosslinker to the particle synthesis makes it possible to perform the crosslinking with an excess or a deficit of the crosslinker. This allows for varying numbers of cationic groups per cationic core and its crosslinking density while the stability of the carrier system remains. The obtained cationic nanohydrogel particles are narrowly distributed in size as determined by dynamic light scattering measurements. Zeta potential measurements confirm the cationic nature of these carriers (ζ > +30 mV) and the ability to form complex anionic siRNA (agarose gel electrophoresis). As a result, it becomes possible to increase the siRNA loading by a factor of four by varying the composition of the crosslinked core. Such an increased siRNA loading should lead to an improved therapeutic gene knockdown effect.	en_GB
dc.language.iso	eng	de
dc.rights	CC BY-NC	*
dc.rights.uri	https://creativecommons.org/licenses/by-nc/4.0/	*
dc.subject.ddc	540 Chemie	de_DE
dc.subject.ddc	540 Chemistry and allied sciences	en_GB
dc.title	Improved SiRNA loading of cationic nanohydrogel particles by variation of crosslinking density	en_GB
dc.type	Zeitschriftenaufsatz	de
dc.identifier.doi	http://doi.org/10.25358/openscience-7011	-
jgu.type.dinitype	article	en_GB
jgu.type.version	Published version	de
jgu.type.resource	Text	de
jgu.organisation.department	FB 09 Chemie, Pharmazie u. Geowissensch.	de
jgu.organisation.number	7950	-
jgu.organisation.name	Johannes Gutenberg-Universität Mainz	-
jgu.rights.accessrights	openAccess	-
jgu.journal.title	Macromolecular chemistry and physics	de
jgu.journal.volume	221	de
jgu.journal.issue	2	de
jgu.pages.alternative	1900298	de
jgu.publisher.year	2020	-
jgu.publisher.name	Wiley-VCH	de
jgu.publisher.place	Weinheim	de
jgu.publisher.issn	1521-3935	de
jgu.organisation.place	Mainz	-
jgu.subject.ddccode	540	de
jgu.publisher.doi	10.1002/macp.201900298	de
jgu.organisation.ror	https://ror.org/023b0x485
Appears in collections:	JGU-Publikationen

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