Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-6493
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dc.contributor.authorNeuberger, Elmo W. I.-
dc.contributor.authorHillen, Barlo-
dc.contributor.authorMayr, Katharina-
dc.contributor.authorSimon, Perikles-
dc.contributor.authorKrämer-Albers, Eva Maria-
dc.contributor.authorBrahmer, Alexandra-
dc.date.accessioned2021-11-12T11:16:37Z-
dc.date.available2021-11-12T11:16:37Z-
dc.date.issued2021-
dc.identifier.urihttps://openscience.ub.uni-mainz.de/handle/20.500.12030/6503-
dc.description.abstractAlthough it is widely accepted that cancer-derived extracellular vesicles (EVs) carry DNA cargo, the association of cell-free circulating DNA (cfDNA) and EVs in plasma of healthy humans remains elusive. Using a physiological exercise model, where EVs and cfDNA are synchronously released, we aimed to characterize the kinetics and localization of DNA associated with EVs. EVs were separated from human plasma using size exclusion chromatography or immuno-affinity capture for CD9+, CD63+, and CD81+ EVs. DNA was quantified with an ultra-sensitive qPCR assay targeting repetitive LINE elements, with or without DNase digestion. This model shows that a minute part of circulating cell-free DNA is associated with EVs. During rest and following exercise, only 0.12% of the total cfDNA occurs in association with CD9+/CD63+/CD81+EVs. DNase digestion experiments indicate that the largest part of EV associated DNA is sensitive to DNase digestion and only ~20% are protected within the lumen of the separated EVs. A single bout of running or cycling exercise increases the levels of EVs, cfDNA, and EV-associated DNA. While EV surface DNA is increasing, DNAse-resistant DNA remains at resting levels, indicating that EVs released during exercise (ExerVs) do not contain DNA. Consequently, DNA is largely associated with the outer surface of circulating EVs. ExerVs recruit cfDNA to their corona, but do not carry DNA in their lumen.en_GB
dc.description.sponsorshipOpen Access-Publizieren Universität Mainz / Universitätsmedizin Mainzde
dc.language.isoengde
dc.rightsCC BY*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subject.ddc570 Biowissenschaftende_DE
dc.subject.ddc570 Life sciencesen_GB
dc.subject.ddc796 Sportde_DE
dc.subject.ddc796 Athletic and outdoor sports and gamesen_GB
dc.titleKinetics and topology of DNA associated with circulating extracellular vesicles released during exerciseen_GB
dc.typeZeitschriftenaufsatzde
dc.identifier.doihttp://doi.org/10.25358/openscience-6493-
jgu.type.dinitypearticleen_GB
jgu.type.versionPublished versionde
jgu.type.resourceTextde
jgu.organisation.departmentFB 02 Sozialwiss., Medien u. Sportde
jgu.organisation.number7910-
jgu.organisation.nameJohannes Gutenberg-Universität Mainz-
jgu.rights.accessrightsopenAccess-
jgu.journal.titleGenesde
jgu.journal.volume12de
jgu.journal.issue4de
jgu.pages.alternative522de
jgu.publisher.year2021-
jgu.publisher.nameMDPIde
jgu.publisher.placeBaselde
jgu.publisher.urihttps://doi.org/10.3390/genes12040522de
jgu.publisher.issn2073-4425de
jgu.organisation.placeMainz-
jgu.subject.ddccode570de
jgu.subject.ddccode796de
jgu.publisher.doi10.3390/genes12040522
Appears in collections:JGU-Publikationen

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