Gutenberg Open Science: Kinetics and topology of DNA associated with circulating extracellular vesicles released during exercise

Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-6493

Full metadata record

DC Field	Value	Language
dc.contributor.author	Neuberger, Elmo W. I.	-
dc.contributor.author	Hillen, Barlo	-
dc.contributor.author	Mayr, Katharina	-
dc.contributor.author	Simon, Perikles	-
dc.contributor.author	Krämer-Albers, Eva-Maria	-
dc.contributor.author	Brahmer, Alexandra	-
dc.date.accessioned	2021-11-12T11:16:37Z	-
dc.date.available	2021-11-12T11:16:37Z	-
dc.date.issued	2021	-
dc.identifier.uri	https://openscience.ub.uni-mainz.de/handle/20.500.12030/6503	-
dc.description.abstract	Although it is widely accepted that cancer-derived extracellular vesicles (EVs) carry DNA cargo, the association of cell-free circulating DNA (cfDNA) and EVs in plasma of healthy humans remains elusive. Using a physiological exercise model, where EVs and cfDNA are synchronously released, we aimed to characterize the kinetics and localization of DNA associated with EVs. EVs were separated from human plasma using size exclusion chromatography or immuno-affinity capture for CD9+, CD63+, and CD81+ EVs. DNA was quantified with an ultra-sensitive qPCR assay targeting repetitive LINE elements, with or without DNase digestion. This model shows that a minute part of circulating cell-free DNA is associated with EVs. During rest and following exercise, only 0.12% of the total cfDNA occurs in association with CD9+/CD63+/CD81+EVs. DNase digestion experiments indicate that the largest part of EV associated DNA is sensitive to DNase digestion and only ~20% are protected within the lumen of the separated EVs. A single bout of running or cycling exercise increases the levels of EVs, cfDNA, and EV-associated DNA. While EV surface DNA is increasing, DNAse-resistant DNA remains at resting levels, indicating that EVs released during exercise (ExerVs) do not contain DNA. Consequently, DNA is largely associated with the outer surface of circulating EVs. ExerVs recruit cfDNA to their corona, but do not carry DNA in their lumen.	en_GB
dc.description.sponsorship	Open Access-Publizieren Universität Mainz / Universitätsmedizin Mainz	de
dc.language.iso	eng	de
dc.rights	CC BY	*
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/	*
dc.subject.ddc	570 Biowissenschaften	de_DE
dc.subject.ddc	570 Life sciences	en_GB
dc.subject.ddc	796 Sport	de_DE
dc.subject.ddc	796 Athletic and outdoor sports and games	en_GB
dc.title	Kinetics and topology of DNA associated with circulating extracellular vesicles released during exercise	en_GB
dc.type	Zeitschriftenaufsatz	de
dc.identifier.doi	http://doi.org/10.25358/openscience-6493	-
jgu.type.dinitype	article	en_GB
jgu.type.version	Published version	de
jgu.type.resource	Text	de
jgu.organisation.department	FB 02 Sozialwiss., Medien u. Sport	de
jgu.organisation.number	7910	-
jgu.organisation.name	Johannes Gutenberg-Universität Mainz	-
jgu.rights.accessrights	openAccess	-
jgu.journal.title	Genes	de
jgu.journal.volume	12	de
jgu.journal.issue	4	de
jgu.pages.alternative	522	de
jgu.publisher.year	2021	-
jgu.publisher.name	MDPI	de
jgu.publisher.place	Basel	de
jgu.publisher.uri	https://doi.org/10.3390/genes12040522	de
jgu.publisher.issn	2073-4425	de
jgu.organisation.place	Mainz	-
jgu.subject.ddccode	570	de
jgu.subject.ddccode	796	de
jgu.publisher.doi	10.3390/genes12040522	-
jgu.organisation.ror	https://ror.org/023b0x485	-
Appears in collections:	JGU-Publikationen

Files in This Item:

	File	Description	Size	Format
	neuberger_elmo-kinetics_and_t-20211112120608184.pdf		10.04 MB	Adobe PDF	View/Open

Show simple item record