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Authors: Neuberger, Elmo W. I.
Hillen, Barlo
Mayr, Katharina
Simon, Perikles
Krämer-Albers, Eva-Maria
Brahmer, Alexandra
Title: Kinetics and topology of DNA associated with circulating extracellular vesicles released during exercise
Online publication date: 12-Nov-2021
Year of first publication: 2021
Language: english
Abstract: Although it is widely accepted that cancer-derived extracellular vesicles (EVs) carry DNA cargo, the association of cell-free circulating DNA (cfDNA) and EVs in plasma of healthy humans remains elusive. Using a physiological exercise model, where EVs and cfDNA are synchronously released, we aimed to characterize the kinetics and localization of DNA associated with EVs. EVs were separated from human plasma using size exclusion chromatography or immuno-affinity capture for CD9+, CD63+, and CD81+ EVs. DNA was quantified with an ultra-sensitive qPCR assay targeting repetitive LINE elements, with or without DNase digestion. This model shows that a minute part of circulating cell-free DNA is associated with EVs. During rest and following exercise, only 0.12% of the total cfDNA occurs in association with CD9+/CD63+/CD81+EVs. DNase digestion experiments indicate that the largest part of EV associated DNA is sensitive to DNase digestion and only ~20% are protected within the lumen of the separated EVs. A single bout of running or cycling exercise increases the levels of EVs, cfDNA, and EV-associated DNA. While EV surface DNA is increasing, DNAse-resistant DNA remains at resting levels, indicating that EVs released during exercise (ExerVs) do not contain DNA. Consequently, DNA is largely associated with the outer surface of circulating EVs. ExerVs recruit cfDNA to their corona, but do not carry DNA in their lumen.
DDC: 570 Biowissenschaften
570 Life sciences
796 Sport
796 Athletic and outdoor sports and games
Institution: Johannes Gutenberg-Universität Mainz
Department: FB 02 Sozialwiss., Medien u. Sport
Place: Mainz
Version: Published version
Publication type: Zeitschriftenaufsatz
License: CC BY
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Journal: Genes
Pages or article number: 522
Publisher: MDPI
Publisher place: Basel
Issue date: 2021
ISSN: 2073-4425
Publisher URL:
Publisher DOI: 10.3390/genes12040522
Appears in collections:JGU-Publikationen

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