Gutenberg Open Science: Polymeric nanoparticles with neglectable protein corona

Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-6260

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DC Field	Value	Language
dc.contributor.author	Alberg, Irina	-
dc.contributor.author	Kramer, Stefan	-
dc.contributor.author	Schinnerer, Meike	-
dc.contributor.author	Hu, Qizhi	-
dc.contributor.author	Seidl, Christine	-
dc.contributor.author	Leps, Christian	-
dc.contributor.author	Drude, Natascha	-
dc.contributor.author	Möckel, Diana	-
dc.contributor.author	Rijcken, Cristianne	-
dc.contributor.author	Lammers, Twan	-
dc.contributor.author	Diken, Mustafa	-
dc.contributor.author	Maskos, Michael	-
dc.contributor.author	Morsbach, Svenja	-
dc.contributor.author	Landfester, Katharina	-
dc.contributor.author	Tenzer, Stefan	-
dc.contributor.author	Barz, Matthias	-
dc.contributor.author	Zentel, Rudolf	-
dc.date.accessioned	2021-08-12T09:12:36Z	-
dc.date.available	2021-08-12T09:12:36Z	-
dc.date.issued	2020	-
dc.identifier.uri	https://openscience.ub.uni-mainz.de/handle/20.500.12030/6270	-
dc.description.abstract	The current understanding of nanoparticle–protein interactions indicates that they rapidly adsorb proteins upon introduction into a living organism. The formed protein corona determines thereafter identity and fate of nanoparticles in the body. The present study evaluates the protein affinity of three core-crosslinked polymeric nanoparticles with long circulation times, differing in the hydrophilic polymer material forming the particle surface, namely poly(N-2-hydroxypropylmethacrylamide) (pHPMA), polysarcosine (pSar), and poly(ethylene glycol) (PEG). This includes the nanotherapeutic CPC634, which is currently in clinical phase II evaluation. To investigate possible protein corona formation, the nanoparticles are incubated in human blood plasma and separated by asymmetrical flow field-flow fractionation (AF4). Notably, light scattering shows no detectable differences in particle size or polydispersity upon incubation with plasma for all nanoparticles, while in gel electrophoresis, minor amounts of proteins can be detected in the particle fraction. Label-free quantitative proteomics is additionally applied to analyze and quantify the composition of the proteins. It proves that some proteins are enriched, but their concentration is significantly less than one protein per particle. Thus, most of the nanoparticles are not associated with any proteins. Therefore, this work underlines that polymeric nanoparticles can be synthesized, for which a protein corona formation does not take place.	en_GB
dc.language.iso	eng	de
dc.rights	CC BY-NC	*
dc.rights.uri	https://creativecommons.org/licenses/by-nc/4.0/	*
dc.subject.ddc	540 Chemie	de_DE
dc.subject.ddc	540 Chemistry and allied sciences	en_GB
dc.subject.ddc	610 Medizin	de_DE
dc.subject.ddc	610 Medical sciences	en_GB
dc.title	Polymeric nanoparticles with neglectable protein corona	en_GB
dc.type	Zeitschriftenaufsatz	de
dc.identifier.doi	http://doi.org/10.25358/openscience-6260	-
jgu.type.dinitype	article	en_GB
jgu.type.version	Published version	de
jgu.type.resource	Text	de
jgu.organisation.department	FB 09 Chemie, Pharmazie u. Geowissensch.	de
jgu.organisation.number	7950	-
jgu.organisation.name	Johannes Gutenberg-Universität Mainz	-
jgu.rights.accessrights	openAccess	-
jgu.journal.title	Small : nano micro	de
jgu.journal.volume	16	de
jgu.journal.issue	18	de
jgu.pages.alternative	1907574	de
jgu.publisher.year	2020	-
jgu.publisher.name	Wiley-VCH	de
jgu.publisher.place	Weinheim	de
jgu.publisher.uri	https://doi.org/10.1002/smll.201907574	de
jgu.publisher.issn	1613-6829	de
jgu.organisation.place	Mainz	-
jgu.subject.ddccode	540	de
jgu.subject.ddccode	610	de
jgu.publisher.doi	10.1002/smll.201907574
jgu.organisation.ror	https://ror.org/023b0x485
Appears in collections:	JGU-Publikationen

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