Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-6260
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dc.contributor.authorAlberg, Irina-
dc.contributor.authorKramer, Stefan-
dc.contributor.authorSchinnerer, Meike-
dc.contributor.authorHu, Qizhi-
dc.contributor.authorSeidl, Christine-
dc.contributor.authorLeps, Christian-
dc.contributor.authorDrude, Natascha-
dc.contributor.authorMöckel, Diana-
dc.contributor.authorRijcken, Cristianne-
dc.contributor.authorLammers, Twan-
dc.contributor.authorDiken, Mustafa-
dc.contributor.authorMaskos, Michael-
dc.contributor.authorMorsbach, Svenja-
dc.contributor.authorLandfester, Katharina-
dc.contributor.authorTenzer, Stefan-
dc.contributor.authorBarz, Matthias-
dc.contributor.authorZentel, Rudolf-
dc.date.accessioned2021-08-12T09:12:36Z-
dc.date.available2021-08-12T09:12:36Z-
dc.date.issued2020-
dc.identifier.urihttps://openscience.ub.uni-mainz.de/handle/20.500.12030/6270-
dc.description.abstractThe current understanding of nanoparticle–protein interactions indicates that they rapidly adsorb proteins upon introduction into a living organism. The formed protein corona determines thereafter identity and fate of nanoparticles in the body. The present study evaluates the protein affinity of three core-crosslinked polymeric nanoparticles with long circulation times, differing in the hydrophilic polymer material forming the particle surface, namely poly(N-2-hydroxypropylmethacrylamide) (pHPMA), polysarcosine (pSar), and poly(ethylene glycol) (PEG). This includes the nanotherapeutic CPC634, which is currently in clinical phase II evaluation. To investigate possible protein corona formation, the nanoparticles are incubated in human blood plasma and separated by asymmetrical flow field-flow fractionation (AF4). Notably, light scattering shows no detectable differences in particle size or polydispersity upon incubation with plasma for all nanoparticles, while in gel electrophoresis, minor amounts of proteins can be detected in the particle fraction. Label-free quantitative proteomics is additionally applied to analyze and quantify the composition of the proteins. It proves that some proteins are enriched, but their concentration is significantly less than one protein per particle. Thus, most of the nanoparticles are not associated with any proteins. Therefore, this work underlines that polymeric nanoparticles can be synthesized, for which a protein corona formation does not take place.en_GB
dc.language.isoengde
dc.rightsCC BY-NC*
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/*
dc.subject.ddc540 Chemiede_DE
dc.subject.ddc540 Chemistry and allied sciencesen_GB
dc.subject.ddc610 Medizinde_DE
dc.subject.ddc610 Medical sciencesen_GB
dc.titlePolymeric nanoparticles with neglectable protein coronaen_GB
dc.typeZeitschriftenaufsatzde
dc.identifier.doihttp://doi.org/10.25358/openscience-6260-
jgu.type.dinitypearticleen_GB
jgu.type.versionPublished versionde
jgu.type.resourceTextde
jgu.organisation.departmentFB 09 Chemie, Pharmazie u. Geowissensch.de
jgu.organisation.number7950-
jgu.organisation.nameJohannes Gutenberg-Universität Mainz-
jgu.rights.accessrightsopenAccess-
jgu.journal.titleSmall : nano microde
jgu.journal.volume16de
jgu.journal.issue18de
jgu.pages.alternative1907574de
jgu.publisher.year2020-
jgu.publisher.nameWiley-VCHde
jgu.publisher.placeWeinheimde
jgu.publisher.urihttps://doi.org/10.1002/smll.201907574de
jgu.publisher.issn1613-6829de
jgu.organisation.placeMainz-
jgu.subject.ddccode540de
jgu.subject.ddccode610de
jgu.publisher.doi10.1002/smll.201907574
Appears in collections:JGU-Publikationen

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