Gutenberg Open Science: Detecting the effects of the glucocorticoid dexamethasone on primary human skeletal muscle cells : differences to the murine cell line

Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-5091

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DC Field	Value	Language
dc.contributor.author	Langendorf, Eva K.	-
dc.contributor.author	Rommens, Pol M.	-
dc.contributor.author	Drees, Philipp	-
dc.contributor.author	Mattyasovszky, Stefan G.	-
dc.contributor.author	Ritz, Ulrike	-
dc.date.accessioned	2020-08-28T09:24:56Z	-
dc.date.available	2020-08-28T09:24:56Z	-
dc.date.issued	2020	-
dc.identifier.uri	https://openscience.ub.uni-mainz.de/handle/20.500.12030/5095	-
dc.description.abstract	Skeletal muscle atrophy is characterized by a decrease in muscle fiber size as a result of a decreased protein synthesis, which leads to degradation of contractile muscle fibers. It can occur after denervation and immobilization, and glucocorticoids (GCs) may also increase protein breakdown contributing to the loss of muscle mass and myofibrillar proteins. GCs are already used in vitro to induce atrophic conditions, but until now no studies with primary human skeletal muscle existed. Therefore, this study deals with the effects of the GC dexamethasone (dex) on primary human myoblasts and myotubes. After incubation with 1, 10, and 100 µM dex for 48 and 72 h, gene and protein expression analyses were performed by qPCR and Western blot. Foxo, MuRF-1, and MAFbx were significantly upregulated by dex, and there was increased gene expression of myogenic markers. However, prolonged incubation periods demonstrated no Myosin protein degradation, but an increase of MuRF-1 expression. In conclusion, applying dex did not only differently affect primary human myoblasts and myotubes, as differences were also observed when compared to murine cells. Based on our findings, studies using cell lines or animal cells should be interpreted with caution as signaling transduction and functional behavior might differ in diverse species. Keywords: atrophy; MuRF-1; myotubes; myoblasts; MAFbx; glucocorticoids; dexamethasone; Foxo; Myosin	en_GB
dc.description.sponsorship	DFG, Open Access-Publizieren Universität Mainz / Universitätsmedizin Mainz	de
dc.language.iso	eng	de
dc.rights	CC BY	de_DE
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/	-
dc.subject.ddc	540 Chemie	de_DE
dc.subject.ddc	540 Chemistry and allied sciences	en_GB
dc.subject.ddc	570 Biowissenschaften	de_DE
dc.subject.ddc	570 Life sciences	en_GB
dc.subject.ddc	610 Medizin	de_DE
dc.subject.ddc	610 Medical sciences	en_GB
dc.title	Detecting the effects of the glucocorticoid dexamethasone on primary human skeletal muscle cells : differences to the murine cell line	en_GB
dc.type	Zeitschriftenaufsatz	de
dc.identifier.doi	http://doi.org/10.25358/openscience-5091	-
jgu.type.contenttype	Scientific article	de
jgu.type.dinitype	article	en_GB
jgu.type.version	Published version	de
jgu.type.resource	Text	de
jgu.organisation.department	FB 04 Medizin	de
jgu.organisation.number	2700	-
jgu.organisation.name	Johannes Gutenberg-Universität Mainz	-
jgu.rights.accessrights	openAccess	-
jgu.journal.title	International journal of molecular sciences	de
jgu.journal.volume	21	de
jgu.journal.issue	7	de
jgu.pages.alternative	2497	de
jgu.publisher.year	2020	-
jgu.publisher.name	MDPI	de
jgu.publisher.place	Basel	-
jgu.publisher.uri	https://doi.org/10.3390/ijms21072497	de
jgu.publisher.issn	1422-0067	de
jgu.organisation.place	Mainz	-
jgu.subject.ddccode	540	de
jgu.subject.ddccode	570	de
jgu.subject.ddccode	610	de
jgu.publisher.doi	10.3390/ijms21072497
jgu.organisation.ror	https://ror.org/023b0x485
Appears in collections:	JGU-Publikationen

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