Please use this identifier to cite or link to this item: http://doi.org/10.25358/openscience-5091
Full metadata record
DC FieldValueLanguage
dc.contributor.authorLangendorf, Eva K.-
dc.contributor.authorRommens, Pol M.-
dc.contributor.authorDrees, Philipp-
dc.contributor.authorMattyasovszky, Stefan G.-
dc.contributor.authorRitz, Ulrike-
dc.date.accessioned2020-08-28T09:24:56Z-
dc.date.available2020-08-28T09:24:56Z-
dc.date.issued2020-
dc.identifier.urihttps://openscience.ub.uni-mainz.de/handle/20.500.12030/5095-
dc.description.abstractSkeletal muscle atrophy is characterized by a decrease in muscle fiber size as a result of a decreased protein synthesis, which leads to degradation of contractile muscle fibers. It can occur after denervation and immobilization, and glucocorticoids (GCs) may also increase protein breakdown contributing to the loss of muscle mass and myofibrillar proteins. GCs are already used in vitro to induce atrophic conditions, but until now no studies with primary human skeletal muscle existed. Therefore, this study deals with the effects of the GC dexamethasone (dex) on primary human myoblasts and myotubes. After incubation with 1, 10, and 100 µM dex for 48 and 72 h, gene and protein expression analyses were performed by qPCR and Western blot. Foxo, MuRF-1, and MAFbx were significantly upregulated by dex, and there was increased gene expression of myogenic markers. However, prolonged incubation periods demonstrated no Myosin protein degradation, but an increase of MuRF-1 expression. In conclusion, applying dex did not only differently affect primary human myoblasts and myotubes, as differences were also observed when compared to murine cells. Based on our findings, studies using cell lines or animal cells should be interpreted with caution as signaling transduction and functional behavior might differ in diverse species. Keywords: atrophy; MuRF-1; myotubes; myoblasts; MAFbx; glucocorticoids; dexamethasone; Foxo; Myosinen_GB
dc.description.sponsorshipDFG, Open Access-Publizieren Universität Mainz / Universitätsmedizin Mainzde
dc.language.isoengde
dc.rightsCC-BYde_DE
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/-
dc.subject.ddc540 Chemiede_DE
dc.subject.ddc540 Chemistry and allied sciencesen_GB
dc.subject.ddc570 Biowissenschaftende_DE
dc.subject.ddc570 Life sciencesen_GB
dc.subject.ddc610 Medizinde_DE
dc.subject.ddc610 Medical sciencesen_GB
dc.titleDetecting the effects of the glucocorticoid dexamethasone on primary human skeletal muscle cells : differences to the murine cell lineen_GB
dc.typeZeitschriftenaufsatzde
dc.identifier.doihttp://doi.org/10.25358/openscience-5091-
jgu.type.contenttypeScientific articlede
jgu.type.dinitypearticleen_GB
jgu.type.versionPublished versionde
jgu.type.resourceTextde
jgu.organisation.departmentFB 04 Medizinde
jgu.organisation.number2700-
jgu.organisation.nameJohannes Gutenberg-Universität Mainz-
jgu.rights.accessrightsopenAccess-
jgu.journal.titleInternational journal of molecular sciencesde
jgu.journal.volume21de
jgu.journal.issue7de
jgu.pages.alternative2497de
jgu.publisher.year2020-
jgu.publisher.nameMDPIde
jgu.publisher.placeBasel-
jgu.publisher.urihttps://doi.org/10.3390/ijms21072497de
jgu.publisher.issn1422-0067de
jgu.organisation.placeMainz-
jgu.subject.ddccode540de
jgu.subject.ddccode570de
jgu.subject.ddccode610de
Appears in collections:JGU-Publikationen

Files in This Item:
File Description SizeFormat 
langendorf_eva_k.-detecting_the_-20200820172623600.pdf4.43 MBAdobe PDFView/Open